| Optimization of mixotrophic medium components for biomass production and biochemical composition biosynthesis by Chlorella vulgaris using response surface methodology | |
| Department | OSSO国家重点实验室 |
Kong WB(孔维宝)1,2,3; Hua SF(华绍烽)1 ; Cao H(曹海)3; Mu YW(慕钰文)3; Yang H(杨红)3; Song H(宋昊)1,2; Xia CG(夏春谷)1; Kong WB(孔维宝); Xia CG(夏春谷)
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| 2012 | |
| Source Publication | Journal of the Taiwan Institute of Chemical Engineers
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| ISSN | 1876-1070 |
| Volume | 43Issue:3Pages:360-367 |
| Abstract | The design of an optimum and mixotrophic medium for biomass production and biochemical composition biosynthesis by Chlorella vulgaris was attempted by using response surface methodology. Based on the Plackett–Burman design, glucose, MgSO4·7H2O, KNO3 and NaCl were selected from the eleven factors as the most critical mixotrophic nutrients. Subsequently, the selected four factors were investigated by the Box–Behnken design. Results showed that the maximum biomass yield of 4.28 g/L was predicted when the concentrations of glucose, MgSO4·7H2O, KNO3 and NaCl were 25 g/L, 1.33 g/L, 1.30 g/L and 3.02 g/L, respectively. Verification of the optimization showed that the biomass content of C. vulgaris could be enhanced from 0.24 g/L in photoautotrophic SEM and 1.89 g/L in unoptimized mixotrophic medium to 4.24 g/L giving 17.67-fold and 2.24-fold increase in biomass production, respectively. The biochemical composition of C. vulgaris cultured in optimized mixotrophic medium indicated that the biomass can be used as the raw material for biorefinery. |
| Keyword | Biomass Production Biochemical Composition Biosynthesis Response Surface Methodology Mixotrophic Medium Components Chlorella Vulgaris |
| Subject Area | 物理化学与绿色催化 |
| DOI | 10.1016/j.jtice.2011.11.007 |
| Funding Organization | National Science Found for Distinguished Young Scholars of China (Grant No. 20625308);Research Fund for Young Teachers of Northwest Normal University (Grant No. NWNU-LKQN-10-30) |
| Indexed By | SCI |
| If | 2.084 |
| Language | 英语 |
| Funding Project | 均相催化研究组 |
| compositor | 第一作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/3897 |
| Collection | 羰基合成与选择氧化国家重点实验室（OSSO） |
| Corresponding Author | Kong WB(孔维宝); Xia CG(夏春谷) |
| Affiliation | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, State Key Lab Oxo Synth & Select Oxidat, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.NW Normal Univ, Coll Iife Sci, Lanzhou 730070, Peoples R China |
| Recommended Citation GB/T 7714 | Kong WB,Hua SF,Cao H,et al. Optimization of mixotrophic medium components for biomass production and biochemical composition biosynthesis by Chlorella vulgaris using response surface methodology[J]. Journal of the Taiwan Institute of Chemical Engineers,2012,43(3):360-367. |
| APA | Kong WB.,Hua SF.,Cao H.,Mu YW.,Yang H.,...&夏春谷.(2012).Optimization of mixotrophic medium components for biomass production and biochemical composition biosynthesis by Chlorella vulgaris using response surface methodology.Journal of the Taiwan Institute of Chemical Engineers,43(3),360-367. |
| MLA | Kong WB,et al."Optimization of mixotrophic medium components for biomass production and biochemical composition biosynthesis by Chlorella vulgaris using response surface methodology".Journal of the Taiwan Institute of Chemical Engineers 43.3(2012):360-367. |
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| B54.Journal of the T（1966KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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