Highly selective colorimetric detection of putrescine in fish products using o-phthalaldehyde derivatization reaction | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Qi X(祁轩)1,2; Wang WF(王伟峰)1; Wang J(王君)1,2; Yang JL(杨军丽)1; Shi YP(师彦平)1; Yang JL(杨军丽); Shi YP(师彦平) | |
2018 | |
Source Publication | Food Chemistry |
ISSN | 0308-8146 |
Volume | 259Issue:0Pages:245-250 |
Abstract | A highly selective colorimetric detection method for putrescine on the basis of an optimized derivatization reaction was established. With the aids of o-phthalaldehyde (OPA) and thioglycolic acid (TGA), putrescine was derived to become red derivatives (PUT-RD), the form that can be detected qualitatively and quantitatively by visual inspection and UV-vis spectrophotometer at λmax of 490 nm, respectively. Key parameters affecting the experiment were investigated by one-factor-at-a-time and response surface analysis. At the optimal condition, this colorimetric method achieved good linearity at putrescine concentrations ranging from 0.8 to 200 μM with detection limit of 0.44 µM. The method also has good selectivity when common amino acids and inorganic ions, as well as ethylenediamine, 1,3-propanediamine, 1,5-pentanediamine, and 1,6-hexanediamine were used as interferences. The established colorimetric method was successfully employed for the detection of putrescine in 10 commercial fish products. |
Keyword | High Selectivity Colorimetric Detection Putrescine (Put) Fish Products |
Subject Area | 分析化学与药物化学 |
DOI | 10.1016/j.foodchem.2018.03.131 |
Funding Organization | the National Natural Science Foundation of China – China (Nos. 81673325 ; 81711540311 ; 21375136 ; 21575150) ; the CAS Pioneer Hundred Talents Program ; the scientific research project of Central Asia Drug Discovery and Development Centre of Chinese Academy of Sciences –China (No. CAM 201704) ; the International Partnership Program of Chinese Academy of Sciences (No. 153631KYSB20160004) |
Indexed By | SCI |
If | 4.946 |
Language | 英语 |
Funding Project | 药物化学成分研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/23634 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Yang JL(杨军丽); Shi YP(师彦平) |
Affiliation | 1.CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, PR China 2.University of Chinese Academy of Sciences, Beijing 100049, PR China |
Recommended Citation GB/T 7714 | Qi X,Wang WF,Wang J,et al. Highly selective colorimetric detection of putrescine in fish products using o-phthalaldehyde derivatization reaction[J]. Food Chemistry,2018,259(0):245-250. |
APA | Qi X.,Wang WF.,Wang J.,Yang JL.,Shi YP.,...&Shi YP.(2018).Highly selective colorimetric detection of putrescine in fish products using o-phthalaldehyde derivatization reaction.Food Chemistry,259(0),245-250. |
MLA | Qi X,et al."Highly selective colorimetric detection of putrescine in fish products using o-phthalaldehyde derivatization reaction".Food Chemistry 259.0(2018):245-250. |
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