α-Glucosidase immobilization on chitosan-enriched magnetic composites for enzyme inhibitors screening | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Liu DM(刘冬梅)1,2; Chen J(陈娟)1; Shi YP(师彦平)1; Chen J(陈娟); Shi YP(师彦平) | |
2017 | |
Source Publication | International Journal of Biological Macromolecules |
ISSN | 0141-8130 |
Volume | 105Pages:308-316 |
Abstract | tIn the present study, the chitosan-enriched magnetic composites (MCCs) were prepared by a novel and simple embedding method for the immobilization of α-glucosidase (α-Glu). The immobilized α-Glu could be easily separated from the reaction mixture under an external magnetic field owing to the magnetic support. With the MCCs-immobilized α-Glu, enzyme activity and stability were studied, and enzyme inhibitors were screened from traditional Chinese medicines (TCMs) and vegetables combined with capillary electrophoresis (CE). The MCCs-immobilized α-Glu exhibited enhanced pH and temperature tolerance with unchanged optimum pH and temperature of 4.0 and 60 ℃ comparing with free α-Glu. Reusability of the immobilized α-Glu was significantly improved after immobilization, and it retained 62.2% of its initial activity after 10 repeated cycles. Immobilized α-Glu also showed improved storagestability (84.3 ±1.2% after 35 days of storage at 4 ℃). The kinetic parameter Kmfor immobilized α-Gluwas calculated to be 0.81 mM and the affinity of enzyme towards its substrate was reduced after immo-bilization. Finally, immobilized α-Glu was used to screen enzyme inhibitors from the extracts of TCMs and vegetables. The enhanced pH and temperature tolerance, improved reusability and storage stability of MCCs-immobilized α-Glu make it a promising candidate for biotechnological applications. |
Keyword | Chitosan-enriched Magnetic Composites Immobilization Alpha-glucosidase Kinetic Study Inhibitor Screening |
Subject Area | 分析化学与药物化学 |
DOI | 10.1016/j.ijbiomac.2017.07.045 |
Funding Organization | the National Natural Science Foun-dation of China (Nos. 21375136;21575150);the ScholarProgram of West Light Project, Chinese Academy of Sciences |
Indexed By | SCI |
If | 3.671 |
Language | 英语 |
Funding Project | 药物化学成分研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/22871 |
Collection | 中国科学院材料磨损与防护重点实验室/先进润滑与防护材料研究发展中心 |
Corresponding Author | Chen J(陈娟); Shi YP(师彦平) |
Affiliation | 1.CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China 2.University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, PR China |
Recommended Citation GB/T 7714 | Liu DM,Chen J,Shi YP,et al. α-Glucosidase immobilization on chitosan-enriched magnetic composites for enzyme inhibitors screening[J]. International Journal of Biological Macromolecules,2017,105:308-316. |
APA | Liu DM,Chen J,Shi YP,陈娟,&师彦平.(2017).α-Glucosidase immobilization on chitosan-enriched magnetic composites for enzyme inhibitors screening.International Journal of Biological Macromolecules,105,308-316. |
MLA | Liu DM,et al."α-Glucosidase immobilization on chitosan-enriched magnetic composites for enzyme inhibitors screening".International Journal of Biological Macromolecules 105(2017):308-316. |
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