Boronate-affinity based magnetic molecularly imprinted nanoparticles for the efficient extraction of the model glycoprotein horseradish peroxidase | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Sun XY(孙晓宇)1,2; Ma RT(马润恬)1; Chen J(陈娟)1; Shi YP(师彦平)1; Chen J(陈娟); Shi YP(师彦平) | |
2017 | |
Source Publication | Microchimica Acta |
ISSN | 0026-3672 |
Volume | 184Issue:10Pages:3729-3737 |
Abstract | Magnetic molecularly imprinted nanoparticles (MMIPs) with improved dispersity and an increased number of adsorption sites are described. Uniform silica layers were first deposited on the surface of Fe3O4 nanoparticles (Fe3O4 NPs) in order to improve the dispersity of magnetic nanoparticles. Then, 4-formylphenylboronic acid (FPBA) as functional monomer was immobilized on the magnetic carriers to improve the efficiency of template eluting and rebinding. A thin layer of polyaniline imprinted with horseradish peroxidase (HRP) as a model glycoprotein was then placed on the magnetic nanoparticles to enhance the dispersity of the resultant MMIPs. These exhibit high adsorption capacity (62 mg g−1), a satisfactory imprinting factor ( 3.78) and short adsorption equilibrium time (40 min) toward HRP, and the limit of detection is 18.7 μg L−1. This kind of MMIPs, therefore, is deemed being a useful tool for extracting low-abundance glycoproteins from even complex samples. |
Keyword | Fe3o4 Nanoparticles Mips Improved Dispersity Thin Imprinted Layer Surface Imprinting |
Subject Area | 分析化学与药物化学 |
DOI | 10.1007/s00604-017-2373-2 |
Funding Organization | the National Natural Science Foundation of China (No. 21475142;21611140105);CAS President’s International Fellowship Initiative (SL: 191);the funds for Distinguished Young Scientists of Gansu (1506RJDA281);the top priority program of “One-Three-Five” Strategic Planning of Chinese Academy of Sciences |
Indexed By | SCI |
If | 4.580 |
Language | 英语 |
Funding Project | 药物化学成分研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/22484 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Chen J(陈娟); Shi YP(师彦平) |
Affiliation | 1.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, China 2.University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, People’s Republic of China |
Recommended Citation GB/T 7714 | Sun XY,Ma RT,Chen J,et al. Boronate-affinity based magnetic molecularly imprinted nanoparticles for the efficient extraction of the model glycoprotein horseradish peroxidase[J]. Microchimica Acta,2017,184(10):3729-3737. |
APA | Sun XY,Ma RT,Chen J,Shi YP,陈娟,&师彦平.(2017).Boronate-affinity based magnetic molecularly imprinted nanoparticles for the efficient extraction of the model glycoprotein horseradish peroxidase.Microchimica Acta,184(10),3729-3737. |
MLA | Sun XY,et al."Boronate-affinity based magnetic molecularly imprinted nanoparticles for the efficient extraction of the model glycoprotein horseradish peroxidase".Microchimica Acta 184.10(2017):3729-3737. |
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