Multi-mode application of graphene quantum dots bonded silica stationary phase for high performance liquid chromatography | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Wu Q(武琪)1,2; Sun YM(孙亚明)1,2; Zhang XL(张晓莉)1,2; Zhang X(张霞)1; Dong SQ(董树清)1; Qiu HD(邱洪灯)1; Wang LT(王利涛)1; Zhao L(赵亮)1; Wang LT(王利涛); Zhao L(赵亮) | |
2017 | |
Source Publication | Journal of Chromatography A |
ISSN | 0021-9673 |
Volume | 1492Pages:61-69 |
Abstract | Graphene quantum dots (GQDs), which possess hydrophobic, hydrophilic, π-π stacking and hydrogen bonding properties, have great prospect in HPLC. In this study, a novel GQDs bonded silica stationary phase was prepared and applied in multiple separation modes including normal phase, reversed phase and hydrophilic chromatography mode. Alkaloids, nucleosides and nucleobases were chosen as test compounds to evaluate the separation performance of this column in hydrophilic chromatographic mode. The tested polar compounds achieved baseline separation and the resolutions reached 2.32, 4.62, 7.79, 1.68 for thymidine, uridine, adenosine, cytidine and guanosine. This new column showed satisfactory chromatographic performance for anilines, phenols and polycyclic aromatic hydrocarbons in normal and reversed phase mode. Five anilines were completely separated within 10 min under the condition of mobile phase containing only 10% methanol. The effect of water content, buffer concentration and pH on chromatographic separation was further investigated, founding that this new stationary phase showed a complex retention mechanism of partitioning, adsorption and electrostatic interaction in hydrophilic chromatography mode, and the multiple retention interactions such as π-π stacking and π-π electron-donor-acceptor interaction played an important role during the separation process. This GQDs bonded column, which allows us to adjust appropriate chromatography mode according to the properties of analytes, has possibility in actual application after further research. |
Keyword | Stationary Phases Graphene Quantum Dots Multiple Retention Interactions Hydrophilic Interaction Chromatography |
Subject Area | 分析化学与药物化学 |
DOI | 10.1016/j.chroma.2017.02.067 |
Funding Organization | the National Natural Science Foundation (Nos. 21405162;21405161;20675085);the Key Projects of Urumqi Science and Technology Bureau (Y141320007);the top priority program of “One-Three-Five” Strategic Planning of Lanzhou Institute of Chemical Physics, CAS |
Indexed By | SCI |
If | 3.981 |
Language | 英语 |
Funding Project | 药物手性分离研究组;药物分离材料研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/21729 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Wang LT(王利涛); Zhao L(赵亮) |
Affiliation | 1.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China 2.University of Chinese Academy of Sciences, Beijing 100039, China |
Recommended Citation GB/T 7714 | Wu Q,Sun YM,Zhang XL,et al. Multi-mode application of graphene quantum dots bonded silica stationary phase for high performance liquid chromatography[J]. Journal of Chromatography A,2017,1492:61-69. |
APA | Wu Q.,Sun YM.,Zhang XL.,Zhang X.,Dong SQ.,...&赵亮.(2017).Multi-mode application of graphene quantum dots bonded silica stationary phase for high performance liquid chromatography.Journal of Chromatography A,1492,61-69. |
MLA | Wu Q,et al."Multi-mode application of graphene quantum dots bonded silica stationary phase for high performance liquid chromatography".Journal of Chromatography A 1492(2017):61-69. |
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1-s2.0-S002196731730(2360KB) | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text |
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