Use of O-carboxymethyl chitosan in high-speed counter-current chromatography: a novel additive for a biphasic solvent system | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Liu YJ(刘言娟)1,2; Wang GH(王高红)1,2; Huang XY(黄新异)1; Liu YF(刘永峰)1; Di DL(邸多隆)1; Di DL(邸多隆) | |
2014 | |
Source Publication | New Journal of Chemistry |
ISSN | 1144-0546 |
Volume | 38Issue:3Pages:1150-1157 |
Abstract | A novel additive, O-carboxymethyl chitosan (O-CMC), for high-speed counter-current chromatography (HSCCC) was found and investigated. It was shown that O-CMC can improve peak resolution (Rs). The mechanism of O-CMC as a solvent additive to improve resolution was studied using quercetin, luteolin and kaempferol as model analytes. It was indicated that O-CMC improved resolution not by increasing the retention of the stationary phase but by introducing intermolecular forces: hydrogen bonding interaction and electrostatic interaction. Steviol glycosides (SGs) were separated by analytical HSCCC using a biphasic solvent system composed of n-hexane/n-butanol/water at a volume ratio of 1.5:3.5:5 with O-CMC (1.0 mg mL−1) in the lower phase. HPLC analysis showed that acceptable separation of SGs was achieved by using O-CMC as a solvent additive and the purities of ST, RC and RA were 61.5%, 97.5% and 74.1%, respectively. |
Subject Area | 分析化学与药物化学 |
DOI | 10.1039/c3nj01140b |
Funding Organization | The National Natural Science Foundation of China (NSFC no. 20775083;21175142);Open Fund of Key Laboratory of Chemistry of Northwestern Plant Resources of The Chinese Academy of Sciences (No. CNPR-2011kfkt-02) |
Indexed By | SCI |
If | 3.086 |
Language | 英语 |
Funding Project | 药物工艺标准研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/21258 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Di DL(邸多隆) |
Affiliation | 1.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Chinese Academy of Sciences, Lanzhou Institute of Chemical Physics, No. 18 Tianshui Middle Road, Lanzhou 730000, China 2.Graduate University of the Chinese Academy of Sciences, Beijing 100049, China |
Recommended Citation GB/T 7714 | Liu YJ,Wang GH,Huang XY,et al. Use of O-carboxymethyl chitosan in high-speed counter-current chromatography: a novel additive for a biphasic solvent system[J]. New Journal of Chemistry,2014,38(3):1150-1157. |
APA | Liu YJ,Wang GH,Huang XY,Liu YF,Di DL,&邸多隆.(2014).Use of O-carboxymethyl chitosan in high-speed counter-current chromatography: a novel additive for a biphasic solvent system.New Journal of Chemistry,38(3),1150-1157. |
MLA | Liu YJ,et al."Use of O-carboxymethyl chitosan in high-speed counter-current chromatography: a novel additive for a biphasic solvent system".New Journal of Chemistry 38.3(2014):1150-1157. |
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