Simultaneous Determination of Five Flavonoids in Saussurea mongolica Franch by High-Performance Liquid Chromatography | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Chen, J.; Shi YP(师彦平)![]() ![]() | |
2012 | |
Source Publication | Acta Chromatographica
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ISSN | 1233-2356 |
Volume | 24Issue:3Pages:489-499 |
Abstract | A high-performance liquid chromatographic (HPLC) technique coupled with photodiode array (PDA) detection has been proposed for simultaneous determination of five flavonoids, i.e. quercetin 3-O-β-d-glucopyranoside, quercetin 4′-methoxy-3-O-β-d-galactopyranoside, kaempferol 3-O-β-l-rhamnopyranoside, asebotin, and kaempferol 7-methxoy-3-O-α-l-rhamnopyranoside in extract of the whole plant of Saussurea mongolica Franch. The optimum conditions for separation were achieved on a 4.6 × 250 mm i.d., 5-μm particle, C18 column with acetonitrile and 1% acetic acid (20:80, v/v) as the mobile phase at a flow rate of 1.0 mL min−1. For all the analytes, a good linear regression relationship (r of >0.999) was obtained between peak area and concentration over a relatively wide range. The method was validated for repeatability, precision, stability, and accuracy. Seven different extraction procedures were investigated for preparation of the sample solution. The validated method was successfully applied to simultaneous analysis of these flavonoids in S. mongolica and was found to be simple and efficient. |
Keyword | Phytochemical Analysis High-performance Liquid Chromatography Saussurea Mongolica Flavonoids |
Subject Area | 分析化学与药物化学 |
DOI | 10.1556/AChrom.24.2012.3.11 |
Funding Organization | the West Light Program of Chinese Academy of Sciences (2007);Science and Technology Foundation for Middle-aged Persons and Young People of Gansu Province (No. 0806RJYA002) |
Indexed By | SCI |
If | 0.644 |
Language | 英语 |
Funding Project | 药物化学成分研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/19547 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Shi YP(师彦平) |
Affiliation | Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China |
Recommended Citation GB/T 7714 | Chen, J.,Shi YP,Shi YP. Simultaneous Determination of Five Flavonoids in Saussurea mongolica Franch by High-Performance Liquid Chromatography[J]. Acta Chromatographica,2012,24(3):489-499. |
APA | Chen, J.,Shi YP,&师彦平.(2012).Simultaneous Determination of Five Flavonoids in Saussurea mongolica Franch by High-Performance Liquid Chromatography.Acta Chromatographica,24(3),489-499. |
MLA | Chen, J.,et al."Simultaneous Determination of Five Flavonoids in Saussurea mongolica Franch by High-Performance Liquid Chromatography".Acta Chromatographica 24.3(2012):489-499. |
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achrom.24.2012.3.11.(140KB) | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text |
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