| Molecularly imprinted polymer for the specific solid-phase extraction of kirenol from Siegesbeckia pubescens herbal extract | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Chen FF(陈方方)1,2,3; Wang R(王瑞)1,2 ; Shi YP(师彦平)1,2; Shi YP(师彦平)
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| 2012 | |
| Source Publication | Talanta
 |
| ISSN | 0039-9140 |
| Volume | 89Pages:505-512 |
| Abstract | Molecular imprinted polymers (MIPs) were prepared by thermal polymerization using a non-covalent molecularly imprinting strategy with kirenol as the template, acrylamide (AM) as the functional monomer and ethylene glycol dimethacrylamide (EGDMA) as the cross-linker in the porogen of tetrahydrofuran (THF). The synthesized MIPs were characterized by scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR). Its molecular recognition property was investigated by UV spectrogram. High-pressure liquid chromatography (HPLC) was used for analysis of target analytes. The polymers were evaluated further by batch rebinding experiments, and from the derived isotherms their binding capacity and binding strength were determined. Then the selectivity of the MIPs was checked toward the selected structurally related compounds and the recognition coefficients for kirenol, darutigenol, and ent-2-oxo-15, 16, 19-trihydroxypimar-8(14)-ene (TD) were 2.47, 3.43 and 3.40, respectively. The properties of MIPs for SPE were also evaluated. The results obtained demonstrate that the good imprinting effect and the excellent selectivity of MIPs were obtained. The optimized molecular imprinted SPE procedure was applied to extract kirenol directly from the extracts of the aerial part of Siegesbeckia pubescens herb. A selective extraction of kirenol from traditional Chinese medicine (TCM) was achieved with extraction yield of 80.9%. |
| Keyword | Molecularly Imprinted Polymers Solid Phase Extraction Kirenol Diterpenoids Traditional Chinese Medicines |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.1016/j.talanta.2011.12.080 |
| Funding Organization | National Natural Science Foundation of China (Nos. 21075127;20875095) |
| Indexed By | SCI |
| If | 3.498 |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| compositor | 第一作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/19503 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Shi YP(师彦平) |
| Affiliation | 1.Chinese Acad Sci, Key Lab Chem NW Plant Resources, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Nat Med Gansu Prov, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| Recommended Citation GB/T 7714 | Chen FF,Wang R,Shi YP,et al. Molecularly imprinted polymer for the specific solid-phase extraction of kirenol from Siegesbeckia pubescens herbal extract[J]. Talanta,2012,89:505-512. |
| APA | Chen FF,Wang R,Shi YP,&师彦平.(2012).Molecularly imprinted polymer for the specific solid-phase extraction of kirenol from Siegesbeckia pubescens herbal extract.Talanta,89,505-512. |
| MLA | Chen FF,et al."Molecularly imprinted polymer for the specific solid-phase extraction of kirenol from Siegesbeckia pubescens herbal extract".Talanta 89(2012):505-512. |
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| 1-s2.0-S003991401200（1199KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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