| Biosurfactant production and characterization of Bacillus sp ZG0427 isolated from oil-contaminated soil | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Zhou, Hangcheng1; Chen, Jixiang1; Yang, Zhi1; Qin B(秦波)2 ; Li, Yanlin1; Kong, Xiuqin1
| |
| 2015 | |
| Source Publication | Annals of Microbiology
 |
| ISSN | 1590-4261 |
| Volume | 65Issue:4Pages:2255-2264 |
| Abstract | An effective biosurfactant-producing strain, denoted ZG0427, was isolated from oil-contaminated soil samples collected from an oilfield in northwest China using the drop-collapse test, oil displacement test, and surface tension measurements. The strain was identified as Bacillus sp. based on the results of morphological and biochemical tests and 16S rDNA sequence analysis. The kinetics of cell growth and biosurfactant production were monitored. A maximum biosurfactant production of 2.79 g/L was achieved when the cells were cultivated in fermentation medium containing 2 % (w/v) of glucose as the sole carbon source, with shaking (180 rpm) at 37 °C for 32 h. The extracted biosurfactant reduced the surface tension of deionized water to 24.6 mN/m, with a critical micelle concentration of 50 mg/L, and showed a remarkable ability to reduce both surface tension and emulsification over wide ranges of pH (5.0–12.0), temperature (−70 to 121 °C), and NaCl concentrations (0–10 %, w/v), respectively. The biosurfactant was extracted from the culture supernatant and partially characterized as lipopeptide. |
| Keyword | Biosurfactant Bacillus Sp. Surface Tension Growth Kinetics Stability |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.1007/s13213-015-1066-5 |
| Funding Organization | the National Natural Science Foundation of China (31272694) |
| Indexed By | SCI |
| If | 1.232 |
| Language | 英语 |
| Funding Project | 植物化学生态研究组 |
| compositor | 第二作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/19081 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Chen, Jixiang |
| Affiliation | 1.Lanzhou Univ Technol, Sch Petrochem Engn, Lanzhou 730050, Gansu, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China |
| Recommended Citation GB/T 7714 | Zhou, Hangcheng,Chen, Jixiang,Yang, Zhi,et al. Biosurfactant production and characterization of Bacillus sp ZG0427 isolated from oil-contaminated soil[J]. Annals of Microbiology,2015,65(4):2255-2264. |
| APA | Zhou, Hangcheng,Chen, Jixiang,Yang, Zhi,Qin B,Li, Yanlin,&Kong, Xiuqin.(2015).Biosurfactant production and characterization of Bacillus sp ZG0427 isolated from oil-contaminated soil.Annals of Microbiology,65(4),2255-2264. |
| MLA | Zhou, Hangcheng,et al."Biosurfactant production and characterization of Bacillus sp ZG0427 isolated from oil-contaminated soil".Annals of Microbiology 65.4(2015):2255-2264. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| art%3A10.1007%2Fs132（565KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment