| Graphitic carbon nitrides modified hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Sun YP(孙英佩)1,2,3; Chen J(陈娟)1,2 ; Qi HY(戚欢阳)1,2; Shi YP(师彦平)1,2; Qi HY(戚欢阳); Shi YP(师彦平)
| |
| 2015 | |
| Source Publication | Journal of Chromatography B
 |
| ISSN | 1570-0232 |
| Volume | 1004Pages:53-59 |
| Abstract | An elevated uric acid (UA) in urine or serum can affect renal function and blood pressure, which is an indicator of gout, cardiovascular and renal diseases, hypertension, etc. In this work, a new type of mixed matrix membrane (MMM), based on graphitic carbon nitrides (g-CNs) and hollow fiber (HF), was prepared and combined with solid phase microextraction (SPME) mode to determine UA in urine and serum followed by gas chromatography-mass spectrometry (GC/MS). The porous g-CNs were dispersed in ammonia, and then the exfoliated g-CNs nanosheets were held in the pores of HF by capillary forces and sonification. The prepared g-CNs modified HF (g-CNs-HF) was immersed in biofluid directly to extract UA with SPME mode and the solvent-free mode is convenient for further derivatization and analysis. To achieve the highest extraction efficiency (EF), main extraction and derivatization parameters, such as g-CNs-HF immobilizing time, sonification power and time of extraction, derivatization and desorption time, were optimized. Under the optimum extraction conditions, a favorable linearity of UA was obtained in the range 0.1-200 mu g mL(-1) with correlation coefficients higher than 0.9990, and the average recoveries at three spiked levels of UA in urine and serum ranged from 80.7% to 121.6%, from 84.7% to 101.1%, respectively. The obtained results demonstrated the developed g-CNs-HF-SPME is a simple, rapid, cost-effective, solvent-free method for the analysis of UA in biofluid. |
| Keyword | Uric Acid (Ua) Graphitic Carbon Nitrides (G-cns) Hollow Fiber (Hf) Solid Phase Microextraction (Spme) Gas Chromatography-mass Spectrometry (Gc/ms) |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.1016/j.jchromb.2015.09.025 |
| Funding Organization | the National Nature Science Foundation of China (Nos. 21375136;21105106);the Scientific research project of Central Asia Drug Discovery and Development Centre of Chinese Academy of Sciences (No. CAM201404) |
| Indexed By | SCI |
| If | 2.687 |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| compositor | 第一作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/18978 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Qi HY(戚欢阳); Shi YP(师彦平) |
| Affiliation | 1.Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| Recommended Citation GB/T 7714 | Sun YP,Chen J,Qi HY,et al. Graphitic carbon nitrides modified hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry[J]. Journal of Chromatography B,2015,1004:53-59. |
| APA | Sun YP,Chen J,Qi HY,Shi YP,戚欢阳,&师彦平.(2015).Graphitic carbon nitrides modified hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry.Journal of Chromatography B,1004,53-59. |
| MLA | Sun YP,et al."Graphitic carbon nitrides modified hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry".Journal of Chromatography B 1004(2015):53-59. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 9-JCB-2015(SunYP).pd（1910KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment