Simultaneous Determination of Eight Flavonoids in the Flowers of Matricaria chamomilla by High Performance Liquid Chromatography | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Jie XY(解笑瑜)1,2,3; Chen FF(陈方方)1,2,3; Shi YP(师彦平)1,2; Shi YP(师彦平) | |
2014 | |
Source Publication | Journal of AOAC International |
ISSN | 1060-3271 |
Volume | 97Issue:3Pages:778-783 |
Abstract | An HPLC method was developed for simultaneous determination of five flavones (apigenin, three apigenin 7-O-glucoside acylated derivatives, and luteolin) and three methoxylated flavonols in Matricaria chamomilla. Full validation of the assay was carried out including linearity, LODs, LOQs, precision, repeatability, stability, and accuracy. The results demonstrated that the method developed was simple, accurate, and reliable. Five batches of M. chamomilla samples were determined using the developed method, and total contents of the eight flavonoids ranged from 1.843 to 2.134 mg/g. Among them, the content of apigenin was the highest with values of 0.538 to 0.618 mg/g. In addition, the extract solution from M. chamomilla exhibited a significant dose-dependent inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH) activity, with a 50% inhibition (SC50) at a concentration of 3.06 ± 0.09 mg/mL, and the flavonoids apigenin-7-O-(6″-acetyl)-glucoside, luteolin, apigenin, eupatolitin, and chrysosplenol D played an important role in the antioxidant activities of the extract solution from M. chamomilla. |
Subject Area | 分析化学与药物化学 |
DOI | 10.5740/jaoacint.13-029 |
Funding Organization | National Natural Science Foundation of China (No. 21075127);the Important Directional Project of the Chinese Academy of Sciences (No. KSCX2-EW-R-15) |
Indexed By | SCI |
If | 1.120 |
Language | 英语 |
Funding Project | 药物化学成分研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/6944 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Shi YP(师彦平) |
Affiliation | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
Recommended Citation GB/T 7714 | Jie XY,Chen FF,Shi YP,et al. Simultaneous Determination of Eight Flavonoids in the Flowers of Matricaria chamomilla by High Performance Liquid Chromatography[J]. Journal of AOAC International,2014,97(3):778-783. |
APA | Jie XY,Chen FF,Shi YP,&师彦平.(2014).Simultaneous Determination of Eight Flavonoids in the Flowers of Matricaria chamomilla by High Performance Liquid Chromatography.Journal of AOAC International,97(3),778-783. |
MLA | Jie XY,et al."Simultaneous Determination of Eight Flavonoids in the Flowers of Matricaria chamomilla by High Performance Liquid Chromatography".Journal of AOAC International 97.3(2014):778-783. |
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