| The Methane Monooxygenase Intrinsic Activity of Kinds of Methanotrophs | |
| Department | 羰基合成与选择氧化国家重点实验室 |
Zhang YX(张颖鑫); Xin JY(辛嘉英); Chen LL(陈琳琳); Xia CG(夏春谷)
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| 2009 | |
| Source Publication | Applied Biochemistry and Biotechnology
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| ISSN | 0273-2289 |
| Volume | 157Pages:431-441 |
| Abstract | Methanotrophs have promising applications in the epoxidation of some alkenes and some chlorinated hydrocarbons and in the production of a biopolymer, poly-β-hydroxybutyrate (poly-3-hydroxybutyrate; PHB). In contrast with methane monooxygenase (MMO) activity and ability of PHB synthesis of four kinds of methanotrophic bacteria Methylosinus trichosporium OB3b, M. trichosporium IMV3011, Methylococcus capsulatus HD6T, Methylomonas sp. GYJ3, and the mixture of the four kinds of strains, M. trichosporium OB3b is the highest of the four in the activity of propene epoxidation (10.72 nmol/min mg dry weight of cell [dwc]), the activity of naphthalene oxidation (22.7 mmol/mg dwc), and ability in synthesis of PHB(11% PHB content in per gram dry weight of cell in 84 h). It could be feasible to improve the MMO activity by mixing four kinds of methanotrophs. The MMO activity dramatically decreased when the cellular PHB accumulated in the second stage. The reason for this may be the dilution of the MMO system in the cells with increasing PHB contents. It has been found that the PHB contents at the level of 1–5% are beneficial to the cells for maintenance of MMO epoxidation activity when enough PHB have been accumulated. Moreover, it was also found that high particulate methane monooxygenase activity may contribute to the synthesis of PHB in the cell, which could be used to improve the yield of PHB in methanotrophs. |
| Keyword | Methanotroph Methane Monooxygenase Poly-β-hydroxybutyrate Reducing Equivalent |
| Subject Area | 清洁催化与生物转化 |
| Funding Organization | the Program for New Century Excellent Talents in University (NCET-05-0358);the National Natural Science Foundation of China (20625308);the Chinese Academy of Sciences |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 清洁催化与生物转化研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/596 |
| Collection | 羰基合成与选择氧化国家重点实验室（OSSO） |
| Corresponding Author | Xin JY(辛嘉英); Xia CG(夏春谷) |
| Recommended Citation GB/T 7714 | Zhang YX,Xin JY,Chen LL,et al. The Methane Monooxygenase Intrinsic Activity of Kinds of Methanotrophs[J]. Applied Biochemistry and Biotechnology,2009,157:431-441. |
| APA | 张颖鑫,辛嘉英,陈琳琳,&夏春谷.(2009).The Methane Monooxygenase Intrinsic Activity of Kinds of Methanotrophs.Applied Biochemistry and Biotechnology,157,431-441. |
| MLA | 张颖鑫,et al."The Methane Monooxygenase Intrinsic Activity of Kinds of Methanotrophs".Applied Biochemistry and Biotechnology 157(2009):431-441. |
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