| Characterization of Candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity | |
| Department | 羰基合成与选择氧化国家重点实验室 |
Yong Y(永阳); Bai YX(白永晓); Li YF(李彦峰); Lin L(林雷); Cui YJ(崔艳君); Xia CG(夏春谷)
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| 2008 | |
| Source Publication | Process Biochemistry
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| ISSN | 1359-5113 |
| Volume | 43Pages:1179-1185 |
| Abstract | Magnetic microspheres were prepared by suspension polymerization of glycidyl methacrylate, ethylene glycol dimethacrylate and vinyl acetate in the presence of oleic acid-coated magnetite, and then, alcoholysed to improve their hydrophilicity. Candida rugosa lipase was covalently immobilized on the hydrophilic magnetic microspheres via the active epoxy groups with the activity yield up to 64.2%. The resulting immobilized lipase had better resistance to pH and temperature inactivation in comparison to free lipase, the adaptive pH and temperature ranges of lipase were widened, and it exhibited good thermal stability and reusability. The resistance of immobilized lipase against urea denaturation was also improved, and the immobilized lipase retained 40% of the activity in the presence of 5 M urea whereas free enzyme retained only 8% of activity. Kinetic parameters were also determined for both immobilized and free lipase. The Km value for immobilized (1.03 mg/ml) was higher than that of the free lipase (0.52 mg/ml), whereas the Vmax value was smaller for the immobilized lipase. |
| Keyword | Hydrophilic Magnetic Microspheres Suspension Polymerization Candida Rugosa Lipase Immobilization |
| Subject Area | 物理化学 |
| Funding Organization | the State Key Laboratory for Oxo Synthesis and Selective Oxidation Open Foundation (Grant No. OSSO0602) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 清洁催化与生物转化研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/571 |
| Collection | 羰基合成与选择氧化国家重点实验室（OSSO） |
| Corresponding Author | Li YF(李彦峰) |
| Recommended Citation GB/T 7714 | Yong Y,Bai YX,Li YF,et al. Characterization of Candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity[J]. Process Biochemistry,2008,43:1179-1185. |
| APA | 永阳,白永晓,李彦峰,林雷,崔艳君,&夏春谷.(2008).Characterization of Candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity.Process Biochemistry,43,1179-1185. |
| MLA | 永阳,et al."Characterization of Candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity".Process Biochemistry 43(2008):1179-1185. |
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| B22_Process Biochem.（738KB） | 开放获取 | License | View Application Full Text | |||
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