| A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Wang FX(王凤霞)1,2; Wu N(吴宁)3; Wei JT(魏鉴腾)4,5 ; Liu J(刘佳)3; Zhao J(赵进)3; Ji AG(吉爱国)1; Lin XK(林秀坤)3,6; Lin XK(林秀坤); Ji AG(吉爱国)
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| 2013 | |
| Source Publication | Biochemistry and Cell Biology
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| ISSN | 0829-8211 |
| Volume | 91Issue:4Pages:244-251 |
| Abstract | Eupolyphaga sinensis Walker is an important insect used in Chinese traditional medicine. In this study, we purified a 72-kDa anticancer protein, designated as EPS72, from this species using ammonium sulfate precipitation, ultrafiltration, CM Sepharose Fast Flow cation exchange, Q Sepharose High Performance (HP) anion exchange, Butyl Sepharose HP hydrophobic chromatography, and Superdex 75 gel filtration chromatographic techniques. EPS72 exhibited a potent anticancer activity against the human lung cancer A549 cell line (IC50, 18.76 μg/mL). Further study showed that EPS72 could induce A549 cell detachment and apoptosis, inhibit cell adhesion to fibronectin and collagen IV, and restrain cell migration and invasion. Moreover, EPS72 significantly decreased the expression of β1-integrin. This study suggests that EPS72 could potentially be developed as a novel anticancer therapeutic agent due to its possible antimetastatic activity. |
| Keyword | Anticancer Protein Antimetastatic Activity Eupolyphaga Sinensis Beta 1-integrin Lung Cancer A549 Cells |
| DOI | 10.1139/bcb-2013-0002 |
| Funding Organization | National 863 high technology program of China (No. 2007AA091403;2007AA09Z408) |
| Indexed By | SCI |
| If | 2.350 |
| Language | 英语 |
| Funding Project | 药物工艺标准研究组 |
| compositor | 第四作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/4927 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Lin XK(林秀坤); Ji AG(吉爱国) |
| Affiliation | 1.Shandong Univ, Sch Pharmaceut Sci, Jinan 250012, Shandong, Peoples R China 2.Shandong Acad Sci, Inst Biol Res, Jinan 250014, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 5.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 6.Capital Med Univ, Dept Pharmacol, Beijing 100069, Peoples R China |
| Recommended Citation GB/T 7714 | Wang FX,Wu N,Wei JT,et al. A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells[J]. Biochemistry and Cell Biology,2013,91(4):244-251. |
| APA | Wang FX.,Wu N.,Wei JT.,Liu J.,Zhao J.,...&吉爱国.(2013).A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells.Biochemistry and Cell Biology,91(4),244-251. |
| MLA | Wang FX,et al."A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells".Biochemistry and Cell Biology 91.4(2013):244-251. |
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| A novel protein from（2911KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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