EFFECTIVE ON-LINE COMBINATION OF HIGH SHEAR TECHNIQUE AND CONTINUOUS SAMPLE INJECTION IN HIGH-PERFORMANCE COUNTER-CURRENT CHROMATOGRAPHY FOR ISOLATION AND PURIFICATION OF POLYPHENOLS IN GREEN TEA | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Chen XF(陈小芬)1,2; Feng ZF(冯祖飞)1,2; Huang XY(黄新异)1; Pei D(裴栋)1,3; Di DL(邸多隆)1,3; Di DL(邸多隆) | |
2014 | |
Source Publication | Journal of Liquid Chromatography & Related Technologies |
ISSN | 1082-6076 |
Volume | 37Issue:18Pages:2571-2586 |
Abstract | A new sample preparation method of high shear dispersing emulsifier (HSDE) coupled with continuous sample injection in high-performance counter-current chromatography (HPCCC) was successfully used for the extraction and online isolation of caffeine and two polyphenolic compounds from green tea. The lower phase of hexane-ethyl acetate–methanol–water (1:8:1:8, v/v/v/v) was used as both the HSDE solvent and HPCCC mobile phase. In one time sample loading, 1.5 mg of (-)-epicatechin-3-gallate, 5.0 mg of (-)-epigallocatechin-3-gallate, and 3.1 mg of caffeine with the purity over 95.0% determined by high performance liquid chromatography (HPLC), could be obtained from about 0.1 g of raw material of green tea. Ten time sample injections were realized in HSDE extraction−HPCCC separation process, and the quantity could be magnified about decuple, 13.8 mg of (-)-epicatechin-3-gallate, 46.3 mg of (-)-epigallocatechin-3-gallate, and 28.9 mg of caffeine could obtain from 1.0 g of raw material of green tea. The compounds were analyzed by HPLC and identified by their chromatograms and their retention time compared with that of authentic reference compounds. It was the first time combining HSDE with continuous sample injection in HPCCC for effective isolation and purification of active compounds from natural products. The mechanism of HSDE extraction was illustrated. |
Keyword | Continuous Sample Injection Green Tea High Shear Dispersing Emulsifier High-performance Counter-current Chromatography Polyphenols |
Subject Area | 分析化学与药物化学 |
DOI | 10.1080/10826076.2013.853306 |
Funding Organization | 国家自然科学基金面上项目 (20974116) |
Indexed By | SCI |
If | 0.565 |
Language | 英语 |
Funding Project | 药物工艺标准研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/4904 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Di DL(邸多隆) |
Affiliation | 1.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Chinese Acad Sci, Lanzhou Inst Chem Phys, Ctr Resource Chem & New Mat, Qingdao, Peoples R China |
Recommended Citation GB/T 7714 | Chen XF,Feng ZF,Huang XY,et al. EFFECTIVE ON-LINE COMBINATION OF HIGH SHEAR TECHNIQUE AND CONTINUOUS SAMPLE INJECTION IN HIGH-PERFORMANCE COUNTER-CURRENT CHROMATOGRAPHY FOR ISOLATION AND PURIFICATION OF POLYPHENOLS IN GREEN TEA[J]. Journal of Liquid Chromatography & Related Technologies,2014,37(18):2571-2586. |
APA | Chen XF,Feng ZF,Huang XY,Pei D,Di DL,&邸多隆.(2014).EFFECTIVE ON-LINE COMBINATION OF HIGH SHEAR TECHNIQUE AND CONTINUOUS SAMPLE INJECTION IN HIGH-PERFORMANCE COUNTER-CURRENT CHROMATOGRAPHY FOR ISOLATION AND PURIFICATION OF POLYPHENOLS IN GREEN TEA.Journal of Liquid Chromatography & Related Technologies,37(18),2571-2586. |
MLA | Chen XF,et al."EFFECTIVE ON-LINE COMBINATION OF HIGH SHEAR TECHNIQUE AND CONTINUOUS SAMPLE INJECTION IN HIGH-PERFORMANCE COUNTER-CURRENT CHROMATOGRAPHY FOR ISOLATION AND PURIFICATION OF POLYPHENOLS IN GREEN TEA".Journal of Liquid Chromatography & Related Technologies 37.18(2014):2571-2586. |
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