| Preconcentration of 3-nitrotyrosine in urine by transient isotachophoresis in MEKC | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Ren HX(任海霞)1,2,3 ; Liu X(刘霞)1,2; Jiang SX(蒋生祥)1,2; Jiang SX(蒋生祥)
| |
| 2013 | |
| Source Publication | Journal of Pharmaceutical and Biomedical Analysis
 |
| ISSN | 0731-7085 |
| Volume | 78-79Pages:100-104 |
| Abstract | Transient isotachophoresis (tITP), usually performed in CZE, is a useful on-line sample preconcentration technique for high saline samples. However, only a few papers have applied tITP in MEKC especially SDS system. This study compared tITP in MEKC with that in CZE for 3-nitro-tyrosine (NT) analysis in urine. No sample clean-up was required. Self-stacking occurred simultaneously as chloride in urine acted as a leading ion. HEPES-Tris buffer at pH 8.2 was used as a terminating electrolyte and separation buffer. UV detection wavelength was set at 426 nm. Experiments showed that tITP in MEKC exhibited much higher peak efficiency (up to 1 000 000), and improved 4-fold sensitivity, compared with those by tITP in CZE. The limit of detection was 0.07 mu M for 3-nitrotyrosine in urine. |
| Keyword | Capillary Electrophoresis Ce-uv High-salt Stacking Nitrotyrosine |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.1016/j.jpba.2013.02.002 |
| Funding Organization | the Natural Science Foundation of Gansu province, China (No. 0803RJZ017);the National Science & Technology Major Project, China (2011ZX05010;2011ZX05011004) |
| Indexed By | SCI |
| If | 2.829 |
| Language | 英语 |
| Funding Project | 药物分离材料研究组 |
| compositor | 第一作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/4619 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Jiang SX(蒋生祥) |
| Affiliation | 1.Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, CAS, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Nat Med Gansu Prov, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Ren HX,Liu X,Jiang SX,et al. Preconcentration of 3-nitrotyrosine in urine by transient isotachophoresis in MEKC[J]. Journal of Pharmaceutical and Biomedical Analysis,2013,78-79:100-104. |
| APA | Ren HX,Liu X,Jiang SX,&蒋生祥.(2013).Preconcentration of 3-nitrotyrosine in urine by transient isotachophoresis in MEKC.Journal of Pharmaceutical and Biomedical Analysis,78-79,100-104. |
| MLA | Ren HX,et al."Preconcentration of 3-nitrotyrosine in urine by transient isotachophoresis in MEKC".Journal of Pharmaceutical and Biomedical Analysis 78-79(2013):100-104. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 任海霞-2.pdf（440KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment