| Holistic analysis of seven constituents from five medicinal herbs composing Wuhu powders in a single run by ultra performance liquid chromatography: application to quality control study | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Chen J(陈娟)1 ; Jin L(晋玲)2; Li YD(李应东)2; Shi YP(师彦平)1; Shi YP(师彦平)
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| 2013 | |
| Source Publication | Analytical Methods
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| ISSN | 1759-9660 |
| Volume | 5Issue:24Pages:7058-7065 |
| Abstract | The newly proposed strategy, namely, holistic analysis of multiple constituents from every constituent herb in a single run, was applied for the quality control of traditional Chinese medicine preparation Wuhu powder. Seven bioactive components, i.e., ferulic acid, hydroxysafflor yellow A, prim-O-glucosylcimifugin, 4′-O-β-D-glucosyl-5-O-methylvisamminol, imperatorin, isoimperatorin, and linoleic acid, from 5 medicinal herbs composing Wuhu powder were selected as evaluation markers, and then their quantitative information was obtained in a single run by an UPLC method coupled with photodiode array detection. Optimum separation was achieved on a C18 column (50 × 2.1 mm i.d., 1.7 μm particle) through a gradient delivery of a mixture of acetonitrile and 0.1% aqueous phosphoric acid at a flow rate of 0.3 mL min_1 at 30 ℃. The corresponding maximum absorption wavelengths were selected as the detection wavelengths for each analyte. For all of the analytes, a good linear relationship (r of >0.999) was obtained between peak area and concentration. The method was validated for repeatability, precision, stability, accuracy, and selectivity. The RSD values of retention time and peak area were 0–0.6% and 0.7–1.0% for intra-assay, and 0.1–1.0% and 1.6–1.8% for inter-assay precision. Analysis repeatability and stability (24 h) were all lower than 5.0%. The recovery of the method was 96.1–97.7% with a RSD of 2.4–4.0%. The validated method was applied to quality control of Wuhu powder from different production batches. |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.1039/c3ay40861b |
| Funding Organization | the National Key Technology Research and Development Program of China (no. 2011BAI05B02);the National Natural Science Foundation of China (no. 21105106) |
| Indexed By | SCI |
| If | 1.938 |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| compositor | 第一作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/4584 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Shi YP(师彦平) |
| Affiliation | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 2.Gansu Coll Tradit Chinese Med, Lanzhou 730000, Peoples R China |
| Recommended Citation GB/T 7714 | Chen J,Jin L,Li YD,et al. Holistic analysis of seven constituents from five medicinal herbs composing Wuhu powders in a single run by ultra performance liquid chromatography: application to quality control study[J]. Analytical Methods,2013,5(24):7058-7065. |
| APA | Chen J,Jin L,Li YD,Shi YP,&师彦平.(2013).Holistic analysis of seven constituents from five medicinal herbs composing Wuhu powders in a single run by ultra performance liquid chromatography: application to quality control study.Analytical Methods,5(24),7058-7065. |
| MLA | Chen J,et al."Holistic analysis of seven constituents from five medicinal herbs composing Wuhu powders in a single run by ultra performance liquid chromatography: application to quality control study".Analytical Methods 5.24(2013):7058-7065. |
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| File Name/Size | DocType | Version | Access | License | ||
| AnalMethods-2013(Che（441KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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