Simultaneous determination of Six alkaloids in phellodendron amurense by high-performance liquid chromatography | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Li CD(李彩东)1,2,3; Liu Y(刘勇)1,2; Shi YP(师彦平)1,2; Shi YP(师彦平) | |
2013 | |
Source Publication | Acta Chromatographia |
ISSN | 1233-2356 |
Volume | 25Issue:2Pages:275-285 |
Abstract | A high-performance liquid chromatography (HPLC) method has been developed for simultaneous determination of six alkaloids, i.e., (−)-(R)-platydesmin, noroxyhydrastinine, berberine, skimmianine, canthin-6-one, and pteleine in the herbal medicine of Phellodendron amurense Rupr. The optimal condition for extraction and separation was achieved with a linear mobile phase gradient prepared from 0.1% phosphoric acid and acetonitrile. The LODs and LOQs for the analytes ranged from 0.06 to 0.22 μg mL−1 and from 0.25 to 0.80 μg mL−1, respectively. The optimized method was applied to the determination of alkaloids in P. amurense Rupr. and was found to be efficient. This method can provide a scientific and technical platform to the manufacturers for setting up a quality control standard as well as to the public for quality and safety assurance of the proprietary traditional Chinese medicines. |
Keyword | High-performance Liquid Chromatography Phytochemical Analysis Traditional Chinese Medicine Phellodendron Amurense Alkaloids |
Subject Area | 分析化学与药物化学 |
DOI | 10.1556/AChrom.25.2013.2.5 |
Funding Organization | the National Key Technology Research and Development Program of China (no. 2007BAI37B05);the National Natural Science Foundation of China (nos. 20875095;21075127) |
Indexed By | SCI |
If | 0.485 |
Language | 英语 |
Funding Project | 药物化学成分研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/4583 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Shi YP(师彦平) |
Affiliation | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
Recommended Citation GB/T 7714 | Li CD,Liu Y,Shi YP,et al. Simultaneous determination of Six alkaloids in phellodendron amurense by high-performance liquid chromatography[J]. Acta Chromatographia,2013,25(2):275-285. |
APA | Li CD,Liu Y,Shi YP,&师彦平.(2013).Simultaneous determination of Six alkaloids in phellodendron amurense by high-performance liquid chromatography.Acta Chromatographia,25(2),275-285. |
MLA | Li CD,et al."Simultaneous determination of Six alkaloids in phellodendron amurense by high-performance liquid chromatography".Acta Chromatographia 25.2(2013):275-285. |
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AChrom-2013(LiCD).pd(157KB) | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text |
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