Nardoguaianone L isolated from Nardostachys jatamansi improved the effect of gemcitabine chemotherapy via regulating AGE signaling pathway in SW1990 cells | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Yi-Dan Zheng1,2; Li-Mei Ma1,3; Jin-Jian Lu4; Tian Chai1; Mohammad Reza Naghavi5; Jun-Yi Ma2; Chun-Yan Sang1; Jun-Li Yang1 | |
The second department | 药物化学与新药分子研究团队 |
2022 | |
Source Publication | Molecules |
Issue | 27Pages:6849 |
Abstract | Pancreatic cancer is the seventh leading cause of cancer-related death worldwide and is known as “the king of cancers”. Currently, gemcitabine (GEM) as the clinical drug of choice for chemotherapy of advanced pancreatic cancer has poor drug sensitivity and ineffective chemotherapy. Nardoguaianone L (G-6) is a novel guaiane-type sesquiterpenoid isolated from Nardostachys jatamansi DC., and it exhibits anti-tumor activity. Based on the newly discovered G-6 with anti-pancreatic cancer activity in our laboratory, this paper aimed to evaluate the potential value of the combination of G-6 and GEM in SW1990 cells, including cell viability, cell apoptosis, colony assay and tandem mass tags (TMT) marker-based proteomic technology. These results showed that G-6 combined with GEM significantly inhibited cell viability, and the effect was more obvious than that with single drug. In addition, the use of TMT marker-based proteomic technology demonstrated that the AGE-RAGE signaling pathway was activated after medication-combination. Furthermore, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) assays were used to validate the proteomic results. Finally, apoptosis was detected by flow cytometry. In conclusion, G-6 combined with GEM induced an increase in ROS level and a decrease in MMP in SW1990 cells through the AGE-RAGE signaling pathway, ultimately leading to apoptosis. G-6 improved the effect of GEM chemotherapy and may be used as a potential combination therapy for pancreatic cancer. |
Keyword | pancreatic cancer gemcitabine proteomic AGE-RAGE signaling pathway SW1990 cells |
DOI | 10.3390/molecules27206849 |
If | 4.927 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/29258 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Jun-Yi Ma; Chun-Yan Sang; Jun-Li Yang |
Affiliation | 1.Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences 2.College of Life Science, Northwest Normal University 3.University of Chinese Academy of Sciences, Chinese Academy of Sciences 4.Institute of Chinese Medical Sciences, University of Macau 5.Agricultural & Natural Resources College, University of Tehran |
Recommended Citation GB/T 7714 | Yi-Dan Zheng,Li-Mei Ma,Jin-Jian Lu,et al. Nardoguaianone L isolated from Nardostachys jatamansi improved the effect of gemcitabine chemotherapy via regulating AGE signaling pathway in SW1990 cells[J]. Molecules,2022(27):6849. |
APA | Yi-Dan Zheng.,Li-Mei Ma.,Jin-Jian Lu.,Tian Chai.,Mohammad Reza Naghavi.,...&Jun-Li Yang.(2022).Nardoguaianone L isolated from Nardostachys jatamansi improved the effect of gemcitabine chemotherapy via regulating AGE signaling pathway in SW1990 cells.Molecules(27),6849. |
MLA | Yi-Dan Zheng,et al."Nardoguaianone L isolated from Nardostachys jatamansi improved the effect of gemcitabine chemotherapy via regulating AGE signaling pathway in SW1990 cells".Molecules .27(2022):6849. |
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