Polymeric ionic liquid modified graphene oxide-grafted silica for solid-phase extraction to analyze the excretion-dynamics of flavonoids in urine by Box-Behnken statistical design | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Hou XD(侯秀丹)1,2; Liu SJ(刘淑娟)1; Zhou PP(周盼盼)3; Li J(李锦)1,2; Liu X(刘霞)1; Wang LC(王立成)1; Guo Y(郭勇)1; Wang LC(王立成); Guo Y(郭勇) | |
2016 | |
Source Publication | Journal of Chromatography A |
ISSN | 0021-9673 |
Volume | 1456Pages:10-18 |
Abstract | A solid-phase extraction method for the efficient analysis of the excretion-dynamics of flavonoids in urine was established and described. In this work, in situ surface radical chain-transfer polymerization and in situ anion exchange were utilized to tune the extraction performance of poly(1-vinyl-3-hexylimidazolium bromide)-graphene oxide-grafted silica (poly(VHIm+Br−)@GO@Sil). Graphene oxide (GO) was first coated onto the silica using a layer-by-layer fabrication method, and then the anion of poly(VHIm+Br−)@GO@Sil was changed into hexafluorophosphate (PF6−) by in situ anion exchange. The interaction energies between two PILs and four flavonoids were calculated with the Gaussian09 suite of programs. A Box-Behnken design was used for the optimization of four greatly influential parameters after single-factor experiments to obtain more accurate and precise results. Coupled to high performance liquid chromatography, the poly(VHIm+PF6−)@GO@Sil method showed acceptable extraction recoveries for the four flavonoids, with limits of detection in the range of 0.1–0.5 μg L−1, and wide linear ranges with correlation coefficients (R) ranging from 0.9935 to 0.9987. Under the optimum conditions, the proposed method was applied to analyze the urines collected from a healthy volunteer. The excretion amount-time profiles revealed that 4–15 h was the main excretion time for the detected flavonoids. The results indicated that the newly developed method offered the advantages of being feasible, green and cost-effective, and could be successfully applied to the extraction and enrichment of flavonoids in human body systems allowing the study of the metabolic kinetics. |
Keyword | Solid-phase Extraction Polymeric Ionic Liquid Interaction Energy Response Surface Methodology Excretion-dynamics |
Subject Area | 分析化学与药物化学 |
DOI | 10.1016/j.chroma.2016.05.096 |
Funding Organization | the National Natural Science Foundation of China (21505146;21575148;21575149;21475143) |
Indexed By | SCI |
If | 3.981 |
Language | 英语 |
Funding Project | 药物分离材料研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/22549 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Wang LC(王立成); Guo Y(郭勇) |
Affiliation | 1.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China 2.University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, China 3.State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China |
Recommended Citation GB/T 7714 | Hou XD,Liu SJ,Zhou PP,et al. Polymeric ionic liquid modified graphene oxide-grafted silica for solid-phase extraction to analyze the excretion-dynamics of flavonoids in urine by Box-Behnken statistical design[J]. Journal of Chromatography A,2016,1456:10-18. |
APA | Hou XD.,Liu SJ.,Zhou PP.,Li J.,Liu X.,...&郭勇.(2016).Polymeric ionic liquid modified graphene oxide-grafted silica for solid-phase extraction to analyze the excretion-dynamics of flavonoids in urine by Box-Behnken statistical design.Journal of Chromatography A,1456,10-18. |
MLA | Hou XD,et al."Polymeric ionic liquid modified graphene oxide-grafted silica for solid-phase extraction to analyze the excretion-dynamics of flavonoids in urine by Box-Behnken statistical design".Journal of Chromatography A 1456(2016):10-18. |
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