Evaluation and Application of a Novel Quantitative Antioxidant Activity Assay Based on Cellular Metabolomics | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Wei JT(魏鉴腾)1,2; Hu, Qingping3; Wang, Ningli1,2; Liu, Yewei3; Pei D(裴栋)1,2; Di DL(邸多隆)1,2; Di DL(邸多隆) | |
The second department | 青岛研发中心/青岛市资源化学与新材料研究中心 |
2017 | |
Source Publication | Chromatographia |
ISSN | 0009-5893 |
Volume | 80Issue:4Pages:617-627 |
Abstract | A novel quantitative antioxidant activity assay based on cellular metabolomics, named cellular metabolomics antioxidant activity (CMAA) assay, was established and applied in this paper. CMAA assay evaluates antioxidant activity by considering the change rules of the cellular metabolites of different treatment groups of human liver carcinoma cell line HepG2 cells subjected to oxidative stress. This assay considers cellular metabolites as the main analytical index, principal components analysis (PCA), and orthogonal projections to latent structures discriminant analysis (OPLS-DA) as the main multi- and megavariate data analysis method and vitamin E (VE) as a standard substance to quantify antioxidant activities. The antioxidant activities of flavonoids extracted from Cynomorium songaricum Rupr. (CS) were measured by the CMAA assay. The results showed that the antioxidant activity of flavonoids extracted from CS was successfully evaluated using the CMAA assay, and the antioxidant activity of flavonoids extracted from CS was about 1/5 of the same concentration of VE. It indicated that the proposed approach, as an effective tool for evaluating the antioxidant capacity, was feasible. |
Keyword | Cellular Metabolomics Antioxidant Activity (Cmaa) Assay Hepg2 Cell Antioxidant Activity Flavonoids Cynomorium Songarium Rupr |
Subject Area | 分析化学与药物化学 |
DOI | 10.1007/s10337-017-3256-8 |
Funding Organization | the National Natural Sciences Foundation of China (No. 21505143);Qingdao Science and Technology Project (No. 15-8-2-6-hy);National High Technology Research and Development Program of China (No. 2014AA022203) |
Indexed By | SCI |
If | 1.402 |
Language | 英语 |
Funding Project | 药物工艺标准研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/21804 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 青岛研发中心/青岛市资源化学与新材料研究中心 |
Corresponding Author | Liu, Yewei; Di DL(邸多隆) |
Affiliation | 1.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, People’s Republic of China 2.Center of Resource Chemical & New Material, Qingdao, People’s Republic of China 3.Institute of Nutrition and Food Hygiene, School of Public Health, Lanzhou University, Lanzhou, People’s Republic of China |
Recommended Citation GB/T 7714 | Wei JT,Hu, Qingping,Wang, Ningli,et al. Evaluation and Application of a Novel Quantitative Antioxidant Activity Assay Based on Cellular Metabolomics[J]. Chromatographia,2017,80(4):617-627. |
APA | Wei JT.,Hu, Qingping.,Wang, Ningli.,Liu, Yewei.,Pei D.,...&邸多隆.(2017).Evaluation and Application of a Novel Quantitative Antioxidant Activity Assay Based on Cellular Metabolomics.Chromatographia,80(4),617-627. |
MLA | Wei JT,et al."Evaluation and Application of a Novel Quantitative Antioxidant Activity Assay Based on Cellular Metabolomics".Chromatographia 80.4(2017):617-627. |
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art%3A10.1007%2Fs103(1216KB) | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text |
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