| Preparative separation and purification of Rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Li, Jie1,2; Chen ZB(陈振斌)1,2,3; Di DL(邸多隆)3
| |
| 2012 | |
| Source Publication | Food Chemistry
 |
| ISSN | 0308-8146 |
| Volume | 132Issue:1Pages:268-276 |
| Abstract | The separation and purification of Rebaudioside A (RA) from Stevia Rebaudiana Bertoni crude extracts (Steviosides) by macroporous adsorption resin (MAR) mixed bed were systematically investigated. MAR mixed bed of HPD750-LSA40-LSA30-DS401 was selected due to its better separation degree. Based on the kinetics/thermodynamics experiment of the mixed bed, it was found that the experimental data fitted better to the pseudo-second-order model, and intra-particle diffusion was rate-limiting step. The adsorption isotherm was consistent with IV equilibrium adsorption isotherm classified by Brunauer. Furthermore, the influencing factors for the separation of RA based on HPLC were also investigated. Under the optimal conditions, the separation degree for RA (D-AS) increased from 0.771 to 1.54. Moreover, the experimental results showed that the purity of the obtained product increased from 60% to 97%. |
| Keyword | Separation Rebaudioside a Stevia Rebaudiana Bertoni Steviosides Macroporous Adsorption Resin Mixed Bed |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.1016/j.foodchem.2011.10.077 |
| Funding Organization | the Graduate Tutor Program Funds of the Gansu Provincial Department of Education (Grant No. 1001ZSB002) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物工艺标准研究组 |
| compositor | 第三作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/19340 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Affiliation | 1.Lanzhou Univ Technol, Sch Mat Sci & Engn, Lanzhou 730050, Gansu, Peoples R China 2.Lanzhou Univ Technol, State Key Lab Gansu Adv Nonferrous Met Mat, Lanzhou 730050, Gansu, Peoples R China 3.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Jie,Chen ZB,Di DL. Preparative separation and purification of Rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins[J]. Food Chemistry,2012,132(1):268-276. |
| APA | Li, Jie,Chen ZB,&Di DL.(2012).Preparative separation and purification of Rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins.Food Chemistry,132(1),268-276. |
| MLA | Li, Jie,et al."Preparative separation and purification of Rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins".Food Chemistry 132.1(2012):268-276. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 1-s2.0-S030881461101（474KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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