Nitro-substituted 3,3 '-bis(indolyl)methane-modified silica gel as a sorbent for solid-phase extraction of flavonoids | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Wang N(王娜)1,2,3; Liang XJ(梁晓静)1,2; Li Q(李欠)1,2; Liao Y(廖原)1,2,3; Shao SJ(邵士俊)1,2; Shao SJ(邵士俊) | |
2015 | |
Source Publication | RSC Advances |
ISSN | 2046-2069 |
Volume | 5Issue:20Pages:15500-15506 |
Abstract | A novel solid-phase extraction (SPE) sorbent was synthesized by chemical immobilization of nitro-substituted 3,3′-bis(indolyl)methane onto pure silica. The nitro-substituted 3,3′-bis(indolyl)methane-modified silica was evaluated by elemental analysis (EA) and Fourier transform infrared spectroscopy (FT-IR). Coupled to high performance liquid chromatography (HPLC), the extraction performance of the sorbent was evaluated by using five flavonoids as model analytes. The results showed that the new sorbent could offer multiple intermolecular interactions, such as π–π, hydrophobic, and hydrogen bonding interactions. Several factors including the extraction material, sample solution pH, sample loading rate, eluent type, volume of eluent, elution rate, and volume of sample loading were optimized. Under the optimal conditions, the proposed method was applied for the analysis of five flavonoids in grape juice. Satisfactory linear ranges for flavonoids were obtained in the range of 10–200 ng mL−1 for myricetin, 5–200 ng mL−1 for quercetin and apigenin, and 1–200 ng mL−1 for luteolin and kaempferol, with correlation coefficients (R) ranging from 0.9908 to 0.9996. Limits of detection (LODs) were in the range of 0.5–10 ng mL−1. The recovery values of spiked grape juice ranged from 91.5% to 120.7% with relative standard deviations (RSDs) less than 9.12% (n = 5). As a novel solid-phase extraction sorbent, the nitro-substituted 3,3′-modified silica exhibited a higher extraction efficiency towards the tested compounds than diol-modified silica. |
Subject Area | 分析化学与药物化学 |
DOI | 10.1039/c4ra13861a |
Funding Organization | the National Natural Science Foundation of China (20972170;21275150);the Funds for Distinguished Young Scientists of Gansu (1210RJDA013) |
Indexed By | SCI |
If | 3.289 |
Language | 英语 |
Funding Project | 药物分子识别研究组;药物分离材料研究组 |
compositor | 第一作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/19306 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Corresponding Author | Shao SJ(邵士俊) |
Affiliation | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources CAS, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
Recommended Citation GB/T 7714 | Wang N,Liang XJ,Li Q,et al. Nitro-substituted 3,3 '-bis(indolyl)methane-modified silica gel as a sorbent for solid-phase extraction of flavonoids[J]. RSC Advances,2015,5(20):15500-15506. |
APA | Wang N,Liang XJ,Li Q,Liao Y,Shao SJ,&邵士俊.(2015).Nitro-substituted 3,3 '-bis(indolyl)methane-modified silica gel as a sorbent for solid-phase extraction of flavonoids.RSC Advances,5(20),15500-15506. |
MLA | Wang N,et al."Nitro-substituted 3,3 '-bis(indolyl)methane-modified silica gel as a sorbent for solid-phase extraction of flavonoids".RSC Advances 5.20(2015):15500-15506. |
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