Interaction of Warfarin with Human Serum Albumin and Effect of Ferulic Acid on the Binding | |
Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Li, Qiang1,2; Yang, Wen-yue3; Qu, Ling-ling3; Qi HY(戚欢阳)4; Huang, Yun3; Zhang, Zheng1,2 | |
2014 | |
Source Publication | Journal of Spectroscopy |
ISSN | 2314-4920 |
Abstract | Angelica sinensis (Oliv.) Diels combined treatment with warfarin would increase the risk of bleeding. Ferulic acid is an abundant hydroxycinnamic acid in A. sinensis and warfarin is the most widely used oral anticoagulant. The studies on supermolecular interaction of warfarin with human serum albumin (HSA) and the influence of ferulic acid on the binding would contribute to the understanding of the metabolic processes of warfarin and the effect of ferulic acid. We focus on investigating the effect of warfarin on fluorescence spectrum of human serum albumin (HSA), fluorescence quenching mechanism, binding constant, Hill coefficient, binding mode, and the effect of different ferulic acid concentrations on the binding. Warfarin quenched the intrinsic fluorescence of HSA mainly by static quenching. Accession of ferulic acid reduced the binding of HSA-warfarin. By decreasing binding constant and the Hill coefficient of warfarin with HSA, ferulic acid could improve the plasma concentration of free warfarin, which would increase the risk of bleeding. Warfarin’s free concentration increased at least 50% under the condition of simulated human body. The results indicated that A. sinensis combined treatment with warfarin would increase the risk of bleeding. And the results provide an important theoretical support for warfarin used as oral anticoagulant. |
Subject Area | 分析化学与药物化学 |
DOI | 10.1155/2014/834501 |
Funding Organization | the Natural Science Foundation of Hebei Province (no. 08B033);the Natural Science Foundation of Gansu Province (no. 1010RJZA123);the Fundamental Research Funds for the Central Universities (lzujbky-2011-102) |
Indexed By | SCI |
If | 0.538 |
Language | 英语 |
Funding Project | 药物化学成分研究组 |
compositor | 第四作者单位 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.licp.cn/handle/362003/19243 |
Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Affiliation | 1.Lanzhou Univ, Hosp 1, Ctr Heart, Lanzhou 730000, Gansu, Peoples R China 2.Lanzhou Univ, Clin Med Coll 1, Lanzhou 730000, Gansu, Peoples R China 3.Hebei Med Univ, Pharmaceut Coll, Shijiazhuang 050017, Peoples R China 4.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Gansu, Peoples R China |
Recommended Citation GB/T 7714 | Li, Qiang,Yang, Wen-yue,Qu, Ling-ling,et al. Interaction of Warfarin with Human Serum Albumin and Effect of Ferulic Acid on the Binding[J]. Journal of Spectroscopy,2014. |
APA | Li, Qiang,Yang, Wen-yue,Qu, Ling-ling,Qi HY,Huang, Yun,&Zhang, Zheng.(2014).Interaction of Warfarin with Human Serum Albumin and Effect of Ferulic Acid on the Binding.Journal of Spectroscopy. |
MLA | Li, Qiang,et al."Interaction of Warfarin with Human Serum Albumin and Effect of Ferulic Acid on the Binding".Journal of Spectroscopy (2014). |
Files in This Item: | ||||||
File Name/Size | DocType | Version | Access | License | ||
834501.pdf(3704KB) | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment