| A Calcium(II)-based L-arginine for ATP binding and hydrolysis | |
| Department | 羰基合成与选择氧化国家重点实验室 ; 精细石油化工中间体国家工程研究中心 |
Ma YQ(马彦青); Lv GX(吕功煊)
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| 2008 | |
| Source Publication | Journal of Inorganic and Organometallic Polymers
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| ISSN | 1574-1443 |
| Volume | 18Pages:435-440 |
| Abstract | The supramolecular recognition of Ca(II) and Na-4-tosyl-L-arginine methyl ester hydrochloride (TAME) with ATP were investigated using 1H and 31P NMR spectra. In the Ca(II)–ATP–TAME ternary system, Ca2+ and TAME bind with ATP via the phosphate chain and adenine ring of ATP. The binding forces are mainly electrostatic and cation (Ca2+)–p and p–p stacking interaction. Furthermore, the hydrolysis of ATP catalyzed by Ca(II) and TAME was studied at pH 7.0 and 60 C using 31P NMR spectra. Kinetics studies show that the ATP hydrolysis rate constant is 0.1035 h-1 in the Ca(II)–TAME–ATP ternary system, whereas the value is 8.5 9 10-3 h-1 under the same conditions without TAME and Ca2+. The Ca(II) ions and TAME accelerate the ATP hydrolysis process about 12-fold. The proposed mechanism of ATP hydrolysis catalyzed by Ca2+–TAME occurs through an addition– elimination reaction sequence. These results can help us get more useful information at the molecular level about the key amino acid residue(s) and metal ions that serve as cofactors in the ATPase effect on ATP hydrolysis/synthesis. |
| Keyword | Atp Ca(Ii) Na-4-tosyl-l-arginine Methyl Ester Hydrochloride Recognition Catalytic Hydrolysis |
| Subject Area | 环境催化与清洁能源 |
| Funding Organization | the National Natural Science Foundation of China (No. 90210027) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 环境催化与清洁能源研究组 ; 环境催化与氢能源研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1905 |
| Collection | 羰基合成与选择氧化国家重点实验室（OSSO） 精细石油化工中间体国家工程研究中心（ERC） |
| Corresponding Author | Lv GX(吕功煊) |
| Recommended Citation GB/T 7714 | Ma YQ,Lv GX. A Calcium(II)-based L-arginine for ATP binding and hydrolysis[J]. Journal of Inorganic and Organometallic Polymers,2008,18:435-440. |
| APA | 马彦青,&吕功煊.(2008).A Calcium(II)-based L-arginine for ATP binding and hydrolysis.Journal of Inorganic and Organometallic Polymers,18,435-440. |
| MLA | 马彦青,et al."A Calcium(II)-based L-arginine for ATP binding and hydrolysis".Journal of Inorganic and Organometallic Polymers 18(2008):435-440. |
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| 65_J.Inorg.Organomet（287KB） | 开放获取 | License | View Application Full Text | |||
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