| Quantitative analysis of five toxic alkaloids in Aconitum pendulum using ultra-performance convergence chromatography (UPC2) coupled with mass spectrometry | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
Zhao TJ(赵堂娟)1,2,3; Qi HY(戚欢阳)1,2 ; Chen J(陈娟)1,2; Shi YP(师彦平)1,2; Chen J(陈娟); Shi YP(师彦平)
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| 2015 | |
| Source Publication | RSC Advances
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| ISSN | 2046-2069 |
| Volume | 5Issue:126Pages:103869-103875 |
| Abstract | A rapid and efficient ultra-performance convergence chromatography (UPC2) method coupled with electrospray ionization single quadrupole mass spectrometry (ESI-MS) was developed and validated for the simultaneous quantification of five diester diterpenoid alkaloid constituents (3-acetylaconitine, hypaconitine, deoxyaconitine, mesaconitine, aconitine) in Aconitum pendulum. Optimum separation was achieved on a BEH 2-EP C18 column (2.1 x 150 mm i.d., 1.7 μm particle) with a gradient elution of a mixture of A (supercritical CO2) and B (methanol containing 10 mmol L-1 ammonium acetate) and at a flow rate of 0.8 mL min-1 within 3 minutes. Quantification was performed using mass spectrometry in a positive ion ionization mode and selected ion recording (SIR) mode. The influences of column, modifier, additive, column temperature, and back pressure were investigated. The five alkaloids were identified and quantified using a comparison of retention time, ultraviolet spectrum, molecular ion peak (obtained from a selective ion recording mode) and peak areas with the reference compounds. The method was validated through linearity, limits of detection, limits of quantification, precision, stability, repeatability, and accuracy. The validated method was applied to analyze A. pendulum, which provided a reference for the quality evaluation of A. pendulum. |
| Subject Area | 分析化学与药物化学 |
| DOI | 10.1039/c5ra21233b |
| Funding Organization | the National Natural Science Foundation of China (No. 21375136;21575150);the Scholar Program of West Light Project, Chinese Academy of Sciences |
| Indexed By | SCI |
| If | 3.289 |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| compositor | 第一作者单位 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/18974 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Chen J(陈娟); Shi YP(师彦平) |
| Affiliation | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Zhao TJ,Qi HY,Chen J,et al. Quantitative analysis of five toxic alkaloids in Aconitum pendulum using ultra-performance convergence chromatography (UPC2) coupled with mass spectrometry[J]. RSC Advances,2015,5(126):103869-103875. |
| APA | Zhao TJ,Qi HY,Chen J,Shi YP,陈娟,&师彦平.(2015).Quantitative analysis of five toxic alkaloids in Aconitum pendulum using ultra-performance convergence chromatography (UPC2) coupled with mass spectrometry.RSC Advances,5(126),103869-103875. |
| MLA | Zhao TJ,et al."Quantitative analysis of five toxic alkaloids in Aconitum pendulum using ultra-performance convergence chromatography (UPC2) coupled with mass spectrometry".RSC Advances 5.126(2015):103869-103875. |
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| File Name/Size | DocType | Version | Access | License | ||
| 3-RSC Advance-2015(Z（432KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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