| Ultrasound-assisted ionic liquid dispersive liquid-liquid microextraction coupled with high performance liquid chromatography for sensitive determination of trace celastrol in urine | |
| Department | 甘肃省天然药物重点实验室 |
Sun JN(孙建楠) ; Shi YP(师彦平); Chen J(陈娟)
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| 2011 | |
| Source Publication | Journal of Chromatography B
 |
| ISSN | 1570-0232 |
| Volume | 879Pages:3429-3433 |
| Abstract | Ultrasound-assisted ionic liquid dispersive liquid–liquid microextraction (UA IL-DLLME) coupled with high-performance liquid chromatography (HPLC) has been developed for the determination of celastrol in human urine samples. In the microextraction procedure, ionic liquid (IL) was used as extraction solvent and dispersed into the aqueous sample solution as fine droplets by means of dispersive solvent and ultrasonication which promoted the analyte to migrate into IL phase more easily. Several important parameters affecting the extraction efficiency were studied and optimized, including the type and volume of extraction solvent and dispersive solvent, sample pH, ultrasonication time, cooling time, centrifugation time and salting-out effect. Under the optimized conditions, 110-fold enrichment factor was obtained and the limit of detection (LOD) was 1.6 _g/L at a signal-to-noise ratio of 3. The calibration curve was linear over the range of 10–1000 _g/L for celastrol in human urine sample, with a correlation coefficient of 0.9980. Intra- and inter-assay precision were 0.43% and 2.78%, respectively. The proposed method was successfully applied to the real human urine samples and good spiked recoveries in the range of 93.2–109.3% were obtained. |
| Keyword | Ultrasound-assisted Il-dllme Hplc Celastrol Urine Sample |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | the National Nature Science Foundation of China (Nos. 20875095;21075127;21105106);Nature Science Foundation of Gansu Province (No. 1107RJZA146) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1518 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Chen J(陈娟) |
| Recommended Citation GB/T 7714 | Sun JN,Shi YP,Chen J. Ultrasound-assisted ionic liquid dispersive liquid-liquid microextraction coupled with high performance liquid chromatography for sensitive determination of trace celastrol in urine[J]. Journal of Chromatography B,2011,879:3429-3433. |
| APA | 孙建楠,师彦平,&陈娟.(2011).Ultrasound-assisted ionic liquid dispersive liquid-liquid microextraction coupled with high performance liquid chromatography for sensitive determination of trace celastrol in urine.Journal of Chromatography B,879,3429-3433. |
| MLA | 孙建楠,et al."Ultrasound-assisted ionic liquid dispersive liquid-liquid microextraction coupled with high performance liquid chromatography for sensitive determination of trace celastrol in urine".Journal of Chromatography B 879(2011):3429-3433. |
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