| Chiral high-performance liquid chromatographic separation of the enantiomers of tetrahydropalmatine and tetrahydroberberine, isolated from Corydalis yanhusuo | |
| Department | 甘肃省天然药物重点实验室 |
Zhai ZD(翟宗德); Shi YP(师彦平) ; Wu XM(吴小梅); Luo XP(罗兴平)
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| 2006 | |
| Source Publication | Anal Bioanal Chem
 |
| ISSN | 1618-2642 |
| Volume | 384Pages:939-945 |
| Abstract | HPLC methods have been developed for chiral resolution of the enantiomers of dl-tetrahydropalmatine (THP) and dl-tetrahydroberberine (THB), two active constituents of Corydalis yanhusuo W.T. Wang. On the analytical scale, good baseline separation of the enantiomers was achieved using cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in both normal-phase and polar organic modes. Validation of the analytical methods, including linearity, limits of detection, recovery, and precision, and semipreparative resolution of dl-THP and dl-THB, were achieved with methanol as mobile phase, without any basic additives, in polar organic mode using cellulose tris (3,5-dimethylphenylcarbamate) chiral stationary phases. On the semipreparative scale, small quantities of the individual enantiomers of THP and THB were isolated for study of the chiroptical properties of the individual enantiomers. |
| Keyword | Enantiomer Separation Polysaccharide Carbamate Phase Specific Rotation Circular Dichroism Tetrahydropalmatine Tetrahydroberberine |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | the “Bairen Jihua” Foundation of Chinese Academy of Sciences (CAS) in 2000;the National Natural Science Foundation of China (NSFC No.20475057) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1448 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Shi YP(师彦平) |
| Recommended Citation GB/T 7714 | Zhai ZD,Shi YP,Wu XM,et al. Chiral high-performance liquid chromatographic separation of the enantiomers of tetrahydropalmatine and tetrahydroberberine, isolated from Corydalis yanhusuo[J]. Anal Bioanal Chem,2006,384:939-945. |
| APA | 翟宗德,师彦平,吴小梅,&罗兴平.(2006).Chiral high-performance liquid chromatographic separation of the enantiomers of tetrahydropalmatine and tetrahydroberberine, isolated from Corydalis yanhusuo.Anal Bioanal Chem,384,939-945. |
| MLA | 翟宗德,et al."Chiral high-performance liquid chromatographic separation of the enantiomers of tetrahydropalmatine and tetrahydroberberine, isolated from Corydalis yanhusuo".Anal Bioanal Chem 384(2006):939-945. |
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