5种生物胺的毛细管胶束电动色谱分离
Department甘肃省天然药物重点实验室
乔成栋; 宋平顺; 严祥; 蒋生祥
2007
Source Publication分析化学
ISSN0253-3820
Volume35Issue:1Pages:95-98
Abstract在胶束电动色谱模式下考察了影响生物胺分离的几个因素(如缓冲溶液及SDS浓度、pH、表面活性剂、分离温度及外加电压等)并优化了分离条件, 建立了一种简单快速的利用毛细管胶束电动色谱DAD检测器分离分析生物胺的新方法。当缓冲溶液为40 mmo l/L硼酸缓冲液( pH 8. 5)、68 mm o l/L SDS, 分离电压为25 kV、分离温度为25e 时, 5种生物胺在7 m in内实现了令人满意的基线分离。在优化的条件下, 对其线性范围、检出限和重现性进行了测定。结果表明, 生物胺的迁移时间的重现性< 0. 8%, 面积的重现性< 4%。
Keyword胶束电动色谱 分离 生物胺 5-甲氧基色胺 5-羟基色氨酸 Micellar Electrokinetic Chromatography Separation Biogenic Amines 5-methoxy Tryptamine d L-5-hydroxytryptophan
Subject Area分析化学与药物化学
Funding Organization国家自然科学基金资助(No. 20375045)
Indexed BySCI
Language中文
Funding Project药物分离材料研究组
Document Type期刊论文
Identifierhttp://ir.licp.cn/handle/362003/1423
Collection中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
Corresponding Author蒋生祥
Recommended Citation
GB/T 7714
乔成栋,宋平顺,严祥,等. 5种生物胺的毛细管胶束电动色谱分离[J]. 分析化学,2007,35(1):95-98.
APA 乔成栋,宋平顺,严祥,&蒋生祥.(2007).5种生物胺的毛细管胶束电动色谱分离.分析化学,35(1),95-98.
MLA 乔成栋,et al."5种生物胺的毛细管胶束电动色谱分离".分析化学 35.1(2007):95-98.
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