| Simultaneous determination of flavonoids in Ixeridium gracile by micellar electrokinetic chromatography | |
| Department | 甘肃省天然药物重点实验室 |
Zhang Y(张玉); Chen J(陈娟) ; Ma XM(马雪梅); Shi YP(师彦平)
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| 2007 | |
| Source Publication | J Pharm Biomed Anal
 |
| ISSN | 0731-7085 |
| Volume | 45Pages:742-746 |
| Abstract | A micellar electrokinetic chromatography (MEKC) method has been developed for the quantitative analysis of five flavonoids: luteolin 7-Oglucoside (LG), 2 ,4 -dihydroxy-dihydrochalcone (DD), 2 ,4 -dihydroxy-chalcone (DC), 7-hydroxy-flavanone (HF) and quercetin 3-O-galactoside (QG) in Ixeridium gracile with UV detection at 275 nm. The applied voltage was 25 kV and the capillary temperature was kept constant at 25 ◦C. The effects of buffer pH, the concentration of electrolyte and organic modifier on migration behavior were studied. Optimum separation condition was achieved with 15mM borate, 30mM sodium dodecyl sulfate (SDS) and 10% (v/v) ethanol at pH 10.5. Regression equations showed good linear relationships (correlation coefficients: 0.9984, 0.9991, 0.9994, 0.9995 and 0.9997) between the peak area of each compound and their concentrations. The relative standard deviations (R.S.D.) of the migration time and peak area were less 1.67 and 3.53% (intra-day), and 1.82 and 3.73% (inter-day), respectively, under the optimum separation conditions. The contents of the five active compounds in I. gracile were determined with satisfactory repeatability and recovery. |
| Keyword | Flavonoids Ixeridium Gracile Micellar Electrokinetic Chromatography |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | National Natural Science Foundation of China (No. 20475057);Natural Science Foundation of Gansu Province (No. 3ZS051-A25-077) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1417 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Shi YP(师彦平) |
| Recommended Citation GB/T 7714 | Zhang Y,Chen J,Ma XM,et al. Simultaneous determination of flavonoids in Ixeridium gracile by micellar electrokinetic chromatography[J]. J Pharm Biomed Anal,2007,45:742-746. |
| APA | 张玉,陈娟,马雪梅,&师彦平.(2007).Simultaneous determination of flavonoids in Ixeridium gracile by micellar electrokinetic chromatography.J Pharm Biomed Anal,45,742-746. |
| MLA | 张玉,et al."Simultaneous determination of flavonoids in Ixeridium gracile by micellar electrokinetic chromatography".J Pharm Biomed Anal 45(2007):742-746. |
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