| Separation and quantification of flavonoid in rhododendron anthopogonoides maxim by high performance liquid chromatography | |
| Department | 甘肃省天然药物重点实验室 |
Zhao L(赵亮) ; Ge J(葛晋); Qiao CD(乔成栋); Zhang HL(张红丽); Jiang SX(蒋生祥)
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| 2008 | |
| Source Publication | Acta chromatographica
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| ISSN | 0231-2522 |
| Volume | 1Issue:20Pages:135 |
| Abstract | A high-performance liquid chromatographic (HPLC) method has been developed for separation and quantitative analysis of flavonoid aglycones in Rhododendron anthopogonosides Maxim. Flavonoids in their bound forms were hydrolyzed with acid before HPLC analysis. Analytical samples were pretreated by solid-phase extraction on C18 reversed-phase cartridges. Optimum separation on a 4.6 mm × 250 mm i.d. C18 column was achieved by use of a 52:48 (v/v) mixture of methanol and an aqueous solution of 10 mm citric acid and 1 mm sodium dodecyl sulfate as mobile phase. The flow rate was 1.0 mL min−1 and the detection wavelength 360 nm. Five flavonoids, myricetin, quercetin, luteolin, kaempferol, and isorhamnetin, were separated with high resolution without use of gradient elution. The method was successfully used for efficient qualitycontrol analysis by quantifying flavonoids in R. anthopogonosides. Repeatability tests showed that intra-day and inter-day RSD was <10%. LOD of the five flavonoids were <0.85 μg mL−1. Recovery ranged from 90.2 to 112.5%, with RSD <11.1%. |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | the National Nature Science Foundation of China (Grant 20675085) ;the Chinese Academy of Sciences for financial support from the Major Frontier Projects (KGCX2-SW-213) ;the Program of the Light in China’s Western Region (2003); the Nature Science Foundation of Gansu Province (no. 3ZS041-A25-23). |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物手性分离研究组 ; 药物分离材料研究组 |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1396 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Zhao L(赵亮) |
| Recommended Citation GB/T 7714 | Zhao L,Ge J,Qiao CD,et al. Separation and quantification of flavonoid in rhododendron anthopogonoides maxim by high performance liquid chromatography[J]. Acta chromatographica,2008,1(20):135. |
| APA | 赵亮,葛晋,乔成栋,张红丽,&蒋生祥.(2008).Separation and quantification of flavonoid in rhododendron anthopogonoides maxim by high performance liquid chromatography.Acta chromatographica,1(20),135. |
| MLA | 赵亮,et al."Separation and quantification of flavonoid in rhododendron anthopogonoides maxim by high performance liquid chromatography".Acta chromatographica 1.20(2008):135. |
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