| Determination of Flavonoids in Tibetan Herbal Medicine of Pyrethrum tatsienense by HPLC with Photodiode Array Detection | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
刘霞(小); Yang AM(杨爱梅); Shi YP(师彦平) ; Shi YP(师彦平)
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| 2008 | |
| Source Publication | Journal of Analytical Chemistry
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| ISSN | 1061-9348 |
| Volume | 63Issue:5Pages:472-476 |
| Abstract | A simple, precise, and rapid method was developed for the determination of four flavonoids, apigenin (AP), tricin (TR), luteolin (LU), and 4'-methoxy-tricin (MTR) in the Tibetan herbal medicine of Pyrethrum tatsienense by the high-performance liquid chromatographic technique coupled with photodiode array detection (DAD). A C 18 reversed-phase column and a linear gradient elution system with acetonitrile–tetrahydrofuran– 1% acetic acid as the mobile phase were employed. The method is validated in terms of the limit of detection (LOD), linearity, precision, and accuracy. The LODs were 48 ng/mL for AP, 75 ng/mL for TR, 88 ng/mL for LU, and 59 ng/mL for MTR, respectively. An excellent linear response was observed over the range specified for all analytes, as confirmed by the correlation coefficient with which from 0.9995 to 0.9999. The intraday RSDs ranged from 0.59 to 2.60%, and the interday RSDs were not higher than 0.05. The accuracy of the method was confirmed with an average recovery ranging from 95.9 to 100.0%. Since the extraction conditions can affect the extraction efficiency, nine different extracts of P. tatsienense were analyzed using different extraction solvents and extraction times. The contents of the four flavonoids in the extract of P. tatsienense were successfully determined within 18 min. |
| Subject Area | 药物化学成分研究 |
| Funding Organization | the National Natural Science Foundation of China (NSFC no. 20475057;20372029);the Natural Science Foundation of Gansu Province (project no. 3ZS051-A25-077) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1378 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Shi YP(师彦平) |
| Recommended Citation GB/T 7714 | Liu X,Yang AM,Shi YP,et al. Determination of Flavonoids in Tibetan Herbal Medicine of Pyrethrum tatsienense by HPLC with Photodiode Array Detection[J]. Journal of Analytical Chemistry,2008,63(5):472-476. |
| APA | 刘霞,Yang AM,Shi YP,&师彦平.(2008).Determination of Flavonoids in Tibetan Herbal Medicine of Pyrethrum tatsienense by HPLC with Photodiode Array Detection.Journal of Analytical Chemistry,63(5),472-476. |
| MLA | 刘霞,et al."Determination of Flavonoids in Tibetan Herbal Medicine of Pyrethrum tatsienense by HPLC with Photodiode Array Detection".Journal of Analytical Chemistry 63.5(2008):472-476. |
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