| Determination of a novel diarylheptanoid (Juglanin B) from green walnut husks (Juglans regia L.) in rat plasma by high-performance liquid Chromatography | |
| Department | 甘肃省天然药物重点实验室 |
Huang XY(黄新异) ; Duan QY(段秋燕); Liu JX(柳军玺); Di DL(邸多隆)
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| 2010 | |
| Source Publication | Biomedical Chromatography
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| ISSN | 1099-0801 |
| Volume | 24Issue:3Pages:307-311 |
| Abstract | A simple and reliable analytical method based on high-performance liquid chromatography (HPLC) coupled with a diode array detector (DAD) was developed for the determination of a novel diarylheptanoid (Juglanin B) from green walnut husks (Juglans regia L.) in rat plasma using rhoiptelol as an internal standard. Chromatographic separation was carried out on a Sinochrom ODS-AP C18 column (250 ￥ 4.6 mm i.d., 5 mm) with acetonitrile–10 mM postassium dihydrogen phosphate (pH = 3; 55:45, v/v) as mobile phase, and the detection wavelength was set at 214 nm. The plasma samples were prepared using methanol as protein precipitator. The extraction recovery of Juglanin B ranged from 70.26 to 78.59%, and the calibration curve had a good linearity in the range 0.08–50 mg/mL (r2 = 0.9932). The RSDs of intra- and inter-day precision ranged from 1.19 to 4.92% and 4.35 to 4.54%, respectively. The HPLC-DAD method described is a simple, rapid and reliable method for the determination of Juglanin B level and for use in studies involving pharmacokinetics. |
| Keyword | JugL.ns Regia L. Diarylheptanoid Juglanin b Pharmacokinetics |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | the “Hundred Talents Program”of the Chinese Academy of Sciences (CAS);the National Natural Sciences Foundation of China (NSFC no.20775083) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物工艺标准研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1262 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Di DL(邸多隆) |
| Recommended Citation GB/T 7714 | Huang XY,Duan QY,Liu JX,et al. Determination of a novel diarylheptanoid (Juglanin B) from green walnut husks (Juglans regia L.) in rat plasma by high-performance liquid Chromatography[J]. Biomedical Chromatography,2010,24(3):307-311. |
| APA | 黄新异,段秋燕,柳军玺,&邸多隆.(2010).Determination of a novel diarylheptanoid (Juglanin B) from green walnut husks (Juglans regia L.) in rat plasma by high-performance liquid Chromatography.Biomedical Chromatography,24(3),307-311. |
| MLA | 黄新异,et al."Determination of a novel diarylheptanoid (Juglanin B) from green walnut husks (Juglans regia L.) in rat plasma by high-performance liquid Chromatography".Biomedical Chromatography 24.3(2010):307-311. |
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