| Simultaneous analysis of xanthone glycosides in Halenia elliptica by HPLC-DAD-ESI/MS | |
| Department | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
刘霞(小); Liu Y(刘勇); Chen J(陈娟) ; Shi YP(师彦平); Shi YP(师彦平)
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| 2010 | |
| Source Publication | J. Chromatogr. Sci.
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| ISSN | 0021-9665 |
| Volume | 48Pages:76-80 |
| Abstract | A new, simple, and sensitive high-performance liquid chromatography–diode array detector (HPLC–DAD) method was developed for the simultaneous determination of six major xanthone glycosides in Halenia elliptica. The chemical profile of six xanthone glycosides, including 2,3,5-trimethoxy-1-Oprimeverosyloxyxanthone (Analyte 1), 2,3,4,5-tetramethoxy-1-Oprimeverosyloxyxanthone (Analyte 2), 2,3,5,7-tetramethoxy-1-Oprimeverosyloxyxanthone (Analyte 3), 2,3,7-trimethoxy-1-Oprimeverosyloxyxanthone (Analyte 4), 2,3,4,7-tetramethoxy-1-Oprimeverosyloxy- xanthone (Analyte 5), and 2,3,4,5,7-pentamethoxy-1-O-primeverosyloxy-xanthone (Analyte 6) were acquired by using HPLC–DAD coupled to an electrospray ionization mass spectrometer. The analysis was performed on a Kromasil C18 column (5 μm, 250 ×4.6 mm i.d.), using acetonitrile–H2O (25:75, v/v) as the mobile phase at a flow rate of 0.8 mL/min. Under UV detection at 260 nm, the recoveries of the analytes were in the range of 96.1–100.3%, the LODs were within 0.20 μg/mL, and all the xanthone glycosides showed good linearity (r ≥0.9990) in a relatively wide concentration range; the intra-day relative standard deviations (%) ranged from 0.65 to 1.39%, and the inter-day RSDs% were not higher than 5%. The proposed method is suitable for quantitative and qualitative determination of the xanthone glycosides in H. elliptica. |
| Subject Area | 药物化学成分研究 |
| Funding Organization | the National Natural Science Foundation of China (NSFC No. 20475057);National Key Technology Research and Development Program of China (No. 2007BAI37B05);the Natural Science Foundation of Gansu Province (No. 3ZS051-A25-077) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1259 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Shi YP(师彦平) |
| Recommended Citation GB/T 7714 | Liu X,Liu Y,Chen J,et al. Simultaneous analysis of xanthone glycosides in Halenia elliptica by HPLC-DAD-ESI/MS[J]. J. Chromatogr. Sci.,2010,48:76-80. |
| APA | 刘霞,Liu Y,Chen J,Shi YP,&师彦平.(2010).Simultaneous analysis of xanthone glycosides in Halenia elliptica by HPLC-DAD-ESI/MS.J. Chromatogr. Sci.,48,76-80. |
| MLA | 刘霞,et al."Simultaneous analysis of xanthone glycosides in Halenia elliptica by HPLC-DAD-ESI/MS".J. Chromatogr. Sci. 48(2010):76-80. |
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