| Determination of aconitine, hypaconitine and mesaconitine in urine using hollow fiber liquid-phase microextraction combined with high-performance liquid chromatography | |
| Department | 甘肃省天然药物重点实验室 |
Yang Y(杨阳); Chen J(陈娟) ; Shi YP(师彦平)
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| 2010 | |
| Source Publication | J. Chromatogr. B
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| ISSN | 1570-0232 |
| Pages | 2811-2816 |
| Abstract | Hollow fiber liquid-phase microextraction (HF-LPME) coupled with high-performance liquid chromatography was used to simultaneously determine three Aconitum alkaloids, including aconitine (AC), hypaconitine (HA) and mesaconitine (MA) in human urine sample. Analytes were extracted from 5mL urine sample containing 1.0 mmol/L NaOH into 1-octanol membrane phase impregnated in the pores of hollow fiber wall, and then back extracted into acidified aqueous solution in the lumen of the hollow fiber. After extraction, 10_L of the acceptor phase was analyzed directly by HPLC. In this method, some important extraction parameters, such as organic solvent, extraction time, stirring rate,pHof donor phase and acceptor phase, temperature, and the volume of acceptor phase were optimized. This method provided 98- to 288-fold enrichment factors within 60 min of extraction and good repeatability with RSDs of 0.99–7.22%. The calibration curves were linear over the ranges of 16.0–128.0_g/L for AC, 11.0–88.0_g/L for HA and 8.1–64.8_g/L for MA in human urine sample, with correlation coefficients of 0.9949, 0.9969 and 0.9904, respectively. Limits of detection were from 0.7 to 1.5_g/L, and recoveries from spiked urine sample varied from 84.4% to 106.2% for AC, 77.3% to 85.6% for HA and 90.1% to 100.8% for MA. |
| Keyword | Hollow Fiber Liquid-phase Microextraction High-performance Liquid Chromatography Aconitine Hypaconitine Mesaconitine Urine Sample |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | the National Nature Science Foundation of China (No. 20875095);National Key Technology Research and Development Program of China (No. 2007BAI37B05);West Light Program of Chinese Academy of Sciences (2007) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1197 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Chen J(陈娟); Shi YP(师彦平) |
| Recommended Citation GB/T 7714 | Yang Y,Chen J,Shi YP. Determination of aconitine, hypaconitine and mesaconitine in urine using hollow fiber liquid-phase microextraction combined with high-performance liquid chromatography[J]. J. Chromatogr. B,2010:2811-2816. |
| APA | 杨阳,陈娟,&师彦平.(2010).Determination of aconitine, hypaconitine and mesaconitine in urine using hollow fiber liquid-phase microextraction combined with high-performance liquid chromatography.J. Chromatogr. B,2811-2816. |
| MLA | 杨阳,et al."Determination of aconitine, hypaconitine and mesaconitine in urine using hollow fiber liquid-phase microextraction combined with high-performance liquid chromatography".J. Chromatogr. B (2010):2811-2816. |
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