| Determination of flavonoids in the flowers of Paulownia tomentosa by high-performance liquid chromatography | |
| Department | 甘肃省天然药物重点实验室 |
Chen J(陈娟) ; Liu Y(刘勇); Shi YP(师彦平)
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| 2009 | |
| Source Publication | Journal of Analytical Chemistry
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| ISSN | 1061-9348 |
| Volume | 64Issue:3Pages:282-288 |
| Abstract | A high-performance liquid chromatographic (HPLC) technique coupled with photodiode array PDA) detection was developed for the simultaneous determination of four flavonoids, i.e. apigenin (AP), diplacone(DI), mimulone (MI) and 5,4'-dihydroxy-7,3'-dimethoxyflavanone (DDF) in extracts of the flowers of Paulownia tomentosa.The optimized method was proposed for the separation and detection of the selected constituents,using methanol-1% acetic acid as the mobile phase at a flow rate of 0.8 mL/min, 290 and 267 nm as the detection wavelengths. All the flavonoids showed good linearity in a relatively wide concentration range(r> 0.9999). The detection limits for the analytes ranged from 0.2 to 4.0 ng, at a signal-to-noise ratio of 3. Interand intra-assay accuracy and precision were all lower than 5.0%. The recovery of the method was 95.9–101.9%. Moreover, the optimized HPLC method was employed to analyze the flowers of Paulownia tomentosa. |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | National Key Technology Research and Development Program of China (no. 2007BAI37B05);West Light Program of Chinese Academy of Sciences (2007);Natural Science Foundation of Gansu Province (no. 3ZS051-A25-077) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1172 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Shi YP(师彦平) |
| Recommended Citation GB/T 7714 | Chen J,Liu Y,Shi YP. Determination of flavonoids in the flowers of Paulownia tomentosa by high-performance liquid chromatography[J]. Journal of Analytical Chemistry,2009,64(3):282-288. |
| APA | 陈娟,刘勇,&师彦平.(2009).Determination of flavonoids in the flowers of Paulownia tomentosa by high-performance liquid chromatography.Journal of Analytical Chemistry,64(3),282-288. |
| MLA | 陈娟,et al."Determination of flavonoids in the flowers of Paulownia tomentosa by high-performance liquid chromatography".Journal of Analytical Chemistry 64.3(2009):282-288. |
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