| Method development and validation for simultaneous HPLC analysis of six active components of the Chinese medicine Qin-Bao-Hong antitussive tablet | |
| Department | 甘肃省天然药物重点实验室 |
Chen J(陈娟) ; Wang GY(王国英); Shi YP(师彦平)
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| 2009 | |
| Source Publication | Acta Chromatographica
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| ISSN | 0231-2522 |
| Volume | 21Issue:2Pages:341-354 |
| Abstract | A high-performance liquid chromatographic (HPLC) method coupled with photodiode array (PDA) detection has been developed and validated for simultaneous analysis of six active components (syringin, hyperoside, baicalin, quercetin, baicalein, and farrerol) of the Chinese medicinal preparation Qin-Bao-Hong antitussive tablet. The optimum conditions for separation were achieved on a 3.9 mm × 150 mm i.d., 5-μm particle, C18 column with a linear mobile phase gradient prepared from acetonitrile and 1% acetic acid at a flow rate of 1.0 mL min−1. Because of the different UV characteristics of these compounds, four detection wavelengths were used for the quantitative analysis (265 nm for syringin, 256 nm for hyperoside and quercetin, 277 nm for baicalin and baicalein, and 296 nm for farrerol). For all the analytes a good linear regression relationship (r > 0.999) was obtained between peak area and concentration over a relatively wide range. The method was validated for repeatability, precision, stability, accuracy, selectivity, and robustness. The validated method was successfully applied to simultaneous analysis of these active components in Qin-Bao-Hong antitussive tablet from different production batches. |
| Keyword | High-performance Liquid Chromatography Traditional Chinese Medicine Qin-bao-hong Antitussive Tablet Quality Control |
| Subject Area | 分析化学与药物化学 |
| Funding Organization | the National Key Technology Research and Development Program of China (no. 2007BAI37B05);the West Light Program of the Chinese Academy of Sciences (2007) |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 药物化学成分研究组 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.licp.cn/handle/362003/1171 |
| Collection | 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| Corresponding Author | Shi YP(师彦平) |
| Recommended Citation GB/T 7714 | Chen J,Wang GY,Shi YP. Method development and validation for simultaneous HPLC analysis of six active components of the Chinese medicine Qin-Bao-Hong antitussive tablet[J]. Acta Chromatographica,2009,21(2):341-354. |
| APA | 陈娟,王国英,&师彦平.(2009).Method development and validation for simultaneous HPLC analysis of six active components of the Chinese medicine Qin-Bao-Hong antitussive tablet.Acta Chromatographica,21(2),341-354. |
| MLA | 陈娟,et al."Method development and validation for simultaneous HPLC analysis of six active components of the Chinese medicine Qin-Bao-Hong antitussive tablet".Acta Chromatographica 21.2(2009):341-354. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Method development a（155KB） | 开放获取 | License | View Application Full Text | |||
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